Caco-2细胞中Lathyrane二萜代谢产物的LC/MS/MS分析

中药材 Pub Date : 2016-08-01
Si-li Tang, Ling-ling Zhang, Wen-jing Huang, Hubiao Chen, Jian-ye Zhang
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引用次数: 0

摘要

目的:采用液相色谱/质谱/质谱法鉴定大黄素L1、大黄素L2、大黄素L8和6(17),12(E)-基醇-5,15-二乙酸酯-3-苯乙酸酯在cco -2细胞中的代谢产物。方法:用100 μg/mol乙烷二萜培养Caco-2细胞,分别培养3、6、12 h。然后采集样品,用LC/MS/MS进行纯化和鉴定。结果:大黄素L1的主要代谢产物是酯水解后的两种甲基化产物。大黄素L2、大黄素L8和6(17),12(E)-基醇-5,15-二乙酸酯-3-苯乙酸酯的主要代谢产物是该酯的水解产物。结论:大黄素L1的主要代谢途径是酯的甲基化和水解。大黄素L2、大黄素L8和6(17),12(E)-基醇-5,15-二乙酸酯-3-苯乙酸酯的主要代谢途径是酯的水解。LC/MS/MS可快速、灵敏地鉴定cco -2细胞中大黄素L1、大黄素L2、大黄素L8和6(17),12(E)-基醇-5,15-二乙酸酯-3-苯乙酸酯的代谢产物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[LC/MS/MS Analysis of the Metabolites of Lathyrane Diterpenoids in Caco-2 Cells].

Objective: To identify the metabolites of euphorbetin L1,euphorbetin L2,euphorbetin L8 and 6( 17),12( E)-lathyrol-5,15-diacetate-3-phenylacetate in Caco-2 cells by LC/MS/MS.

Methods: Caco-2 cells were cultured with 100 μg/mol lathyrane diterpenoid for 3,6,12 h,respectively. Then the samples were collected,purified and identified by LC/MS/MS.

Results: The major metabolites of euphorbetin L1 were two methylated products which were obtained after hydrolysis of the ester. The major metabolites of euphorbetin L2,euphorbetin L8 and 6( 17),12( E)-lathyrol-5,15-diacetate-3-phenylacetate were hydrolysis products of the ester.

Conclusion: The main metabolic pathway of euphorbetin L1 is methylation and hydrolysis of the ester. The main metabolic pathway of euphorbetin L2,euphorbetin L8 and 6( 17),12( E)-lathyrol-5,15-diacetate-3-phenylacetate is hydrolysis of the ester. LC/MS/MS can identify the metabolites of euphorbetin L1,euphorbetin L2,euphorbetin L8 and 6( 17),12( E)-lathyrol-5,15-diacetate-3-phenylacetate in Caco-2 cells quickly and sensitively.

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期刊介绍: "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.
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