海胆雌性配子线粒体通透性过渡孔的研究

IF 2.6 Q2 Medicine
Elis Torrezan-Nitao , Regina Celia Bressan Queiroz Figueiredo , Luis Fernando Marques-Santos
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引用次数: 4

摘要

线粒体通透性过渡孔(MPTP)与多种细胞类型的钙稳态和活性氧(ROS)的产生有关。虽然在体细胞中研究广泛,但关于配子中MPTP现象的数据很少。本研究旨在探讨MPTP在海胆雌性配子中的发生情况。以质子载体CCCP、FCCP和Ca2+离子载体离子霉素作为孔诱导剂。采用线粒体电位敏感探针和钴猝灭钙黄蛋白测定法监测孔开度。孔脱敏剂环孢素A (CsA)可防止MPTP激活剂引起的线粒体内膜电位损失(ΔΨm)和孔打开。ΔΨm的破坏导致ROS生成增加,而CsA完全阻止了这一过程。我们的数据还表明,MPTP开放诱导的ROS产生的增加需要细胞外Ca2+。总之,目前的研究提供了证据,证明海胆卵中MPTP的发生方式与脊椎动物体细胞相似- csa敏感,电压和Ca2+触发-并表明MPTP在进化过程中是一个高度保守的生理事件。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Mitochondrial permeability transition pore in sea urchin female gametes

Mitochondrial permeability transition pore in sea urchin female gametes

Mitochondrial permeability transition pore (MPTP) has been associated to calcium homeostasis and reactive oxygen species (ROS) generation in several cell types. While extensively investigated in somatic cells, there are few data regarding MPTP phenomenon in gametes. The aim of the present work was to investigate MPTP occurrence in sea urchin female gametes. The protonophores CCCP and FCCP, and the Ca2+ ionophore ionomycin, were used as pore inductors. Pore opening was monitored by mitochondrial potential sensitive probes and cobalt-quenched calcein assay. The pore desensitizer cyclosporin A (CsA) prevented the loss of mitochondrial inner membrane potential (ΔΨm) and pore opening induced by MPTP activators. The disruption of ΔΨm led to an increase in ROS generation, which was completely prevented by CsA. Our data also demonstrated that the increase in ROS production induced by MPTP opening requires extracellular Ca2+. In summary, the current study provides evidence about the occurrence of MPTP in sea urchin eggs in a similar manner as described in vertebrate somatic cells - CsA-sensitive, voltage- and Ca2+-triggered - and shows MPTP as a highly conserved physiological event through the evolution.

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来源期刊
Mechanisms of Development
Mechanisms of Development 生物-发育生物学
CiteScore
3.60
自引率
0.00%
发文量
0
审稿时长
12.4 weeks
期刊介绍: Mechanisms of Development is an international journal covering the areas of cell biology and developmental biology. In addition to publishing work at the interphase of these two disciplines, we also publish work that is purely cell biology as well as classical developmental biology. Mechanisms of Development will consider papers in any area of cell biology or developmental biology, in any model system like animals and plants, using a variety of approaches, such as cellular, biomechanical, molecular, quantitative, computational and theoretical biology. Areas of particular interest include: Cell and tissue morphogenesis Cell adhesion and migration Cell shape and polarity Biomechanics Theoretical modelling of cell and developmental biology Quantitative biology Stem cell biology Cell differentiation Cell proliferation and cell death Evo-Devo Membrane traffic Metabolic regulation Organ and organoid development Regeneration Mechanisms of Development does not publish descriptive studies of gene expression patterns and molecular screens; for submission of such studies see Gene Expression Patterns.
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