环形毛霉:生长、维持和基因操作

Current Protocols in Microbiology Pub Date : 2018-04-27 DOI:10.1002/cpmc.53

Sandeep Vellanki, Maria Isabel Navarro-Mendoza, Alexis Garcia, Laura Murcia, Carlos Perez-Arques, Victoriano Garre, Francisco E. Nicolas, Soo Chan Lee

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引用次数: 38

摘要

毛霉(Mucor circinelloides)是毛霉目的一种真菌。它在环境中以霉菌的形式生长，并可引起毛霉病，这是免疫功能低下患者的一种潜在致命感染。M. circinelloides是一种生物柴油生产者，是研究光反应和RNA干扰介导的基因沉默等生物过程的模式生物。在过去的十年里，越来越多的分子工具也使我们能够操纵这种真菌的基因组。本文概述了在实验室中体外生长、维持和遗传操作的基本方案。©2018 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Mucor circinelloides: Growth, Maintenance, and Genetic Manipulation

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Mucor circinelloides: Growth, Maintenance, and Genetic Manipulation

Mucor circinelloides is a fungus that belongs to the order Mucorales. It grows as mold in the environment and can cause mucormycosis, a potentially fatal infection in immunocompromised patients. M. circinelloides is a biodiesel producer and serves as a model organism for studying several biological processes, such as light responses and RNA interference–mediated gene silencing. Over the past decade, the increasing number of molecular tools has also allowed us to manipulate the genome of this fungus. This article outlines the fundamental protocols for the in vitro growth, maintenance, and genetic manipulation of M. circinelloides in the laboratory. © 2018 by John Wiley & Sons, Inc.

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Current Protocols in Microbiology Immunology and Microbiology-Parasitology

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期刊介绍： Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.