Ric-8A是异源三聚体g蛋白的GEF,在迁移过程中控制颅神经嵴细胞极性

IF 2.6 Q2 Medicine
Juan Ignacio Leal , Soraya Villaseca , Andrea Beyer , Gabriela Toro-Tapia , Marcela Torrejón
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引用次数: 4

摘要

神经嵴(NC)是一个短暂的胚胎细胞群,在发育过程中广泛迁移。不同Gα亚基的鸟嘌呤核苷酸交换因子(GEF) ricc - 8a调节爪蟾颅内NC (CNC)细胞迁移,其机制尚不清楚。为了正确迁移,CNC细胞建立极化轴,并发生形态变化,在细胞前缘产生突起,细胞后部收缩。本研究以GTPase为基础的活细胞探针,以aPKC和Par3为极性标记物,通过检测其信号传导是否影响GTPase在非洲爪蟾CNC中活性的定位,旨在研究ricc - 8a在CNC细胞迁移过程中对细胞极性的作用。我们发现,在迁移过程中,ric8a的水平至关重要,并影响极性标记物的定位和GTPase活性的亚细胞定位,这表明ric8a可能通过异源三聚体g蛋白信号传导调节CNC迁移过程中的细胞极性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ric-8A, a GEF for heterotrimeric G-proteins, controls cranial neural crest cell polarity during migration

The neural crest (NC) is a transient embryonic cell population that migrates extensively during development. Ric-8A, a guanine nucleotide exchange factor (GEF) for different Gα subunits regulates cranial NC (CNC) cell migration in Xenopus through a mechanism that still remains to be elucidated. To properly migrate, CNC cells establish an axis of polarization and undergo morphological changes to generate protrusions at the leading edge and retraction of the cell rear. Here, we aim to study the role of Ric-8A in cell polarity during CNC cell migration by examining whether its signaling affects the localization of GTPase activity in Xenopus CNC using GTPase-based probes in live cells and aPKC and Par3 as polarity markers. We show that the levels of Ric-8A are critical during migration and affect the localization of polarity markers and the subcellular localization of GTPase activity, suggesting that Ric-8A, probably through heterotrimeric G-protein signaling, regulates cell polarity during CNC migration.

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来源期刊
Mechanisms of Development
Mechanisms of Development 生物-发育生物学
CiteScore
3.60
自引率
0.00%
发文量
0
审稿时长
12.4 weeks
期刊介绍: Mechanisms of Development is an international journal covering the areas of cell biology and developmental biology. In addition to publishing work at the interphase of these two disciplines, we also publish work that is purely cell biology as well as classical developmental biology. Mechanisms of Development will consider papers in any area of cell biology or developmental biology, in any model system like animals and plants, using a variety of approaches, such as cellular, biomechanical, molecular, quantitative, computational and theoretical biology. Areas of particular interest include: Cell and tissue morphogenesis Cell adhesion and migration Cell shape and polarity Biomechanics Theoretical modelling of cell and developmental biology Quantitative biology Stem cell biology Cell differentiation Cell proliferation and cell death Evo-Devo Membrane traffic Metabolic regulation Organ and organoid development Regeneration Mechanisms of Development does not publish descriptive studies of gene expression patterns and molecular screens; for submission of such studies see Gene Expression Patterns.
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