单核细胞和中性粒细胞的分离、迁移和吞噬试验。

Q2 Immunology and Microbiology
Current Protocols in Immunology Pub Date : 2018-08-01 Epub Date: 2018-07-03 DOI:10.1002/cpim.53
Alexander Maini, Juliet R Foote, Richard Hayhoe, Amit A Patel, Alastair O'Brien, Inbal Avraham-Davidi, Simon Yona
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引用次数: 1

摘要

本文描述了分离小鼠单核细胞和中性粒细胞的方法,以及用于测量细胞吞噬、迁移和极化的体外方案。本文所采用的分离原始吞噬细胞的方法克服了以前在吞噬细胞活化方面遇到的许多困难。为分析细胞迁移提供了三种体外方案,一种不需要专门的设备,一种需要改进的Boyden室,另一种采用流动室,测量细胞粘附,滚动和迁移。三个体外方案检查吞噬已包括在这个更新的版本。最后,提出了一种利用共聚焦显微镜对极化细胞进行成像的方法。©2018 by John Wiley & Sons, Inc。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Monocyte and Neutrophil Isolation, Migration, and Phagocytosis Assays.

This article describes methods for isolating mouse monocytes and neutrophils, as well as in vitro protocols for measuring cell phagocytosis, migration, and polarization. The method employed here for the isolation of naive phagocytes overcomes many of the difficulties previously encountered concerning phagocyte activation. Three in vitro protocols are provided for the analysis of cell migration, one requiring no specialized equipment, one requiring a modified Boyden chamber, and the other employing a flow chamber, which measures cell adhesion, rolling, and migration. Three in vitro protocols to examine phagocytosis have been included in this updated version. Finally, a method is provided for imaging polarized cells by confocal microscopy. © 2018 by John Wiley & Sons, Inc.

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来源期刊
Current Protocols in Immunology
Current Protocols in Immunology Immunology and Microbiology-Immunology
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