ENA核苷酸和ENA寡核苷酸的合成

Q4 Chemistry

Current Protocols in Nucleic Acid Chemistry Pub Date : 2018-04-02 DOI:10.1002/cpnc.46

Koji Morita, Makoto Koizumi

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引用次数: 6

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Synthesis of ENA Nucleotides and ENA Oligonucleotides

2′-O,4′-C-Ethylene-bridged nucleic acid (ENA) is a sugar-modified oligonucleotide with an ethylene bridge between the 2′-oxygen and 4′-carbon of ribose. ENA not only has as high binding affinity to complementary RNA as conventional bridged/locked nucleic acid, but also has much higher nuclease resistance in plasma, which makes it a promising candidate for antisense therapeutics. This unit presents detailed protocols for the synthesis and characterization of ENA nucleosides and oligonucleotides. © 2018 by John Wiley & Sons, Inc.

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来源期刊

Current Protocols in Nucleic Acid Chemistry Chemistry-Organic Chemistry

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期刊介绍： Published in association with International Society for Nucleosides, Nucleotides & Nucleic Acids (IS3NA) , Current Protocols in Nucleic Acid Chemistry is equally valuable for biotech, pharmaceutical, and academic labs. It is the resource for designing and running successful research projects in the rapidly growing and changing field of nucleic acid, nucleotide, and nucleoside research.