不同氮浓度和培养基更换对普通小球藻光合微藻细胞生长、脂质生成及生产力的影响

Q1 Agricultural and Biological Sciences
Mohammad H Morowvat, Younes Ghasemi
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引用次数: 2

摘要

背景:普通小球藻(Chlorella vulgaris)是一种光合单细胞微藻,被认为是一种强大的生物质和脂质生产者,可用于人类食物和饲料,也可用于绿色能源生产。培养基组成对生物量和脂质产量和生产力起着至关重要的作用。方法:采用BG11和f/2培养基,分3次培养试验,各培养22 d,研究不同氮水平和培养基更换对光合绿色微藻细胞生长、产脂量和生产力的影响。结果:在f/2培养基中,总脂产量达到0.246 g L-1(41.55%),比BG11培养基(0.298 g L-1)提高了147%。在添加6.00 g硝酸盐的BG11-3培养基中,总叶绿素含量(15.189 mg L-1)和细胞数(3.812 × 106个细胞mL-1)较高,生物量最高(1.492 g L-1)。脂肪酸谱由饱和脂肪酸、单不饱和脂肪酸和多不饱和脂肪酸组成,包括具有营养或燃烧意义的硬脂酸、油酸、α-亚麻酸和γ-亚麻酸。结论:这些数据表明,这种C. vulgaris菌株可能被认为是生物质、脂质和绿色能源生产的强大来源。新建立的专利为通过不同的生物工艺和基因工程方法获得具有改进性能的高脂和脂肪酸的未来方向指明了方向。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cell Growth, Lipid Production and Productivity in Photosynthetic Microalga Chlorella vulgaris under Different Nitrogen Concentrations and Culture Media Replacement.

Background: The photosynthetic unicellular microalgal strain, Chlorella vulgaris is considered as a robust biomass and lipid producer for human food and feed and also green energy production. The culture media composition plays a crucial role in biomass and lipid production and productivity.

Methods: The photosynthetic green microalga C. vulgaris was studied in three distinct cultivation experiments each for 22 days, using BG11 and f/2 culture media to investigate the effects of different levels of nitrogen and culture media replacement on cell growth, lipid production and productivity.

Results: In f/2 culture medium, the total lipid production reached was 0.246 g L-1 (41.55%) which was 147% higher than its counterpart in BG11 medium (0.298 g L-1). On the other hand, the highest biomass (1.492 g L-1) was attained in BG11-3 medium with 6.00 g nitrate L-1 and this was correlated with higher total chlorophyll content (15.189 mg L-1) and cell number (3.812 × 106 cell mL-1). A fatty acid profile, composed of saturated, monounsaturated and polyunsaturated fatty acids was detected and included stearic acid, oleic acid, α-linolenic acid and γ-linolenic acid which have nutritional or combustion importance.

Conclusion: These data suggest that this C. vulgaris strain may be considered as a robust source for biomass, lipid and green energy production. The newly established patents have shed light on the future directions to achieve higher lipids and fatty acids with improved properties through different bioprocess and genetic engineering approaches.

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来源期刊
Recent patents on food, nutrition & agriculture
Recent patents on food, nutrition & agriculture Agricultural and Biological Sciences-Agronomy and Crop Science
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