扩散张量成像对脑白质缺血性损伤的渐进式评估:猕猴脑卒中模型的初步研究。

Q4 Medicine
Open Neuroimaging Journal Pub Date : 2018-03-30 eCollection Date: 2018-01-01 DOI:10.2174/1874440001812010030
Xiaodong Zhang, Yumei Yan, Frank Tong, Chun-Xia Li, Benjamin Jones, Silun Wang, Yuguang Meng, E Chris Muly, Doty Kempf, Leonard Howell
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引用次数: 17

摘要

背景:以往的弥散张量成像(Diffusion Tensor Imaging, DTI)研究表明,脑卒中脑灰质和白质损伤的时间演变可以通过DTI指标来表征。然而,在脑卒中超急性期(前6小时)和急性期(≤1周)白质DTI指数是如何逐渐改变的,目前还不完全清楚。在本研究中,采用DTI表征永久性缺血性闭塞猕猴模型脑卒中后梗死和白质损伤的时间演变。方法与材料:采用恒河猴(n=4, 10 ~ 21岁)永久性大脑中动脉闭塞术。在闭塞后超急性期(2-6小时,n=4)、48小时(n=4)和96小时(n=3),用DTI纵向检查脑病变。结果:所有动物均出现皮质梗死。在第一个时间点(脑卒中后2小时),病变区域的平均弥漫性(MD)显著下降(35%,p)。结论:采用猕猴模型和DTI表征脑卒中后2 ~ 96小时灰质和白质缺血性损伤的时间演变。永久性MCA闭塞后,从超急性期到急性期,白质束的进行性MD变化与整个梗死区域的MD变化在时间上相关。与灰质相比,白质束的MD减少是轻微的,但明显且进行性的,表明它可能有助于检测脑卒中后早期白质变性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Progressive Assessment of Ischemic Injury to White Matter Using Diffusion Tensor Imaging: A Preliminary Study of a Macaque Model of Stroke.

Progressive Assessment of Ischemic Injury to White Matter Using Diffusion Tensor Imaging: A Preliminary Study of a Macaque Model of Stroke.

Progressive Assessment of Ischemic Injury to White Matter Using Diffusion Tensor Imaging: A Preliminary Study of a Macaque Model of Stroke.

Progressive Assessment of Ischemic Injury to White Matter Using Diffusion Tensor Imaging: A Preliminary Study of a Macaque Model of Stroke.

Background: Previous Diffusion Tensor Imaging (DTI) studies have demonstrated the temporal evolution of stroke injury in grey matter and white matter can be characterized by DTI indices. However, it still remains not fully understood how the DTI indices of white matter are altered progressively during the hyperacute (first 6 hours) and acute stage of stroke (≤ 1 week). In the present study, DTI was employed to characterize the temporal evolution of infarction and white matter injury after stroke insult using a macaque model with permanent ischemic occlusion.

Methods and materials: Permanent middle cerebral artery (MCA) occlusion was induced in rhesus monkeys (n=4, 10-21 years old). The brain lesion was examined longitudinally with DTI during the hyperacute phase (2-6 hours, n=4), 48 hours (n=4) and 96 hours (n=3) post-occlusion.

Results: Cortical infarction was seen in all animals. The Mean Diffusivity (MD) in lesion regions decreased substantially at the first time point (2 hours post stroke) (35%, p <0.05, compared to the contralateral side) and became pseudo-normalized at 96 hours. In contrast, evident FA reduction was seen at 48 hours (39%, p <0.10) post-stroke. MD reduction in white matter bundles of the lesion area was much less than that in the grey matter during the hyper-acute phase but significant change was observed 4 hours (4.2%, p < 0.05) post stroke . Also, MD pseudonormalisation was seen at 96 hours post stroke. There was a significant correlation between the temporal changes of MD in white matter bundles and those in whole lesion areas during the entire study period. Meanwhile, no obvious fractional anisotropy (FA) changes were seen during the hyper-acute phase in either the entire infarct region or white matter bundles. Significant FA alteration was observed in entire lesion areas and injured white matter bundles 48 and 96 hours post stroke. The stroke lesion in grey matter and white matter was validated by pathological findings.

Conclusion: The temporal evolution of ischemic injury to the grey matter and white matter from 2 to 96 hours after stroke onset was characterized using a macaque model and DTI. Progressive MD changes in white matter bundles are seen from hyperacute phase to acute phase after permanent MCA occlusion and temporally correlated with the MD changes in entire infarction regions. MD reduction in white matter bundles is mild in comparison with that in the grey matter but significant and progressive, indicating it may be useful to detect early white matter degeneration after stroke.

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来源期刊
Open Neuroimaging Journal
Open Neuroimaging Journal Medicine-Radiology, Nuclear Medicine and Imaging
CiteScore
0.70
自引率
0.00%
发文量
3
期刊介绍: The Open Neuroimaging Journal is an Open Access online journal, which publishes research articles, reviews/mini-reviews, and letters in all important areas of brain function, structure and organization including neuroimaging, neuroradiology, analysis methods, functional MRI acquisition and physics, brain mapping, macroscopic level of brain organization, computational modeling and analysis, structure-function and brain-behavior relationships, anatomy and physiology, psychiatric diseases and disorders of the nervous system, use of imaging to the understanding of brain pathology and brain abnormalities, cognition and aging, social neuroscience, sensorimotor processing, communication and learning.
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