青藏高原察罕盐湖可培养中嗜盐细菌的群落结构和多样性

微生物学报 Pub Date : 2017-04-04

Shuo Shen

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引用次数: 0

摘要

目的:研究可汗盐湖可培养中嗜盐细菌的群落结构和多样性。方法:在不同选择培养基上分离培养中等嗜盐菌。在对16S rRNA基因序列进行扩增和测量后，根据16S rRNA基因信息构建系统发生树，分析群落结构，计算多样性指数。结果:从可汗盐湖水体和泥样中分离出中度嗜盐细菌421株。16S rRNA基因信息显示4个潜在新种属于芽孢杆菌科。83个模式菌株分属3门6科16属。其中，芽孢杆菌、海洋杆菌和盐单胞菌为优势种。多样性分析表明，从水样中分离出的菌株多样性高于泥样，但泥样中分离出的菌株优势度高于水样。结论:可汗盐湖中分离到的中等嗜盐细菌具有丰富的遗传多样性。此外，该湖泊还存在优势种和新种可培养的中等嗜盐细菌。

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[Community structure and diversity of culturable moderate halophilic bacteria isolated from Qrhan salt lake on Qinghai-Tibet Plateau].

Objective: I studied the community structure and diversity of culturable moderate halophilic bacteria isolated from Qrhan Salt Lake.

Methods: I isolated and cultured the moderate halophilic bacteria on different selective media. After the 16S rRNA gene sequences was amplified and measured, I constructed the phylogenic tree, analyzed the community structure and calculated the diversity indexes according to the 16S rRNA gene information.

Results: A total of 421 moderate halophilic bacteria were isolated from water and mud samples in Qrhan Salt Lake. The 16S rRNA gene information showed that 4 potential novel species belonged to the family Bacillaceae. Eighty-three model strains belonged to 3 phylurms 6 families 16 genus. Among them, Bacillus sp., Oceanobacillus sp. and Halomonas sp. were dominant species. Diversity analysis showed that the diversity of strains isolated from water sample was higher than that from mud sample, but the dominance degree of strains isolated from mud sample was higher than that from water sample.

Conclusion: The genetic diversity of moderate halophilic bacteria isolated from Qrhan Salt Lake was abundant. Also, there were dominant and novel species of culturable moderate halophilic bacteria in this lake.

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来源期刊

微生物学报

自引率

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发文量

7960

期刊介绍： Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.