[生防菌株棕榈链霉菌Act12 spa7074缺失突变体的构建及其次生代谢产物的鉴定]。

微生物学报 Pub Date : 2016-12-04

Xuemei Duan, Feiyang Zhao, Xia Yan, Quanhong Xue, Xiaoxia Li, Bingjie Wen, Lianghui Jia, Hua Yan

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引用次数: 0

摘要

目的:破坏生物防治菌株Act12中编码TetR家族转录因子的spa7074，并对其次生代谢产物进行表征。方法:采用同源重组法破坏spa7074基因。利用高效液相色谱法检测突变菌株Δspa7074和Act12的次生代谢产物。用质谱和核磁共振对其结构进行了分析。结果:与野生型菌株相比，突变株Δspa7074中一些未知化合物的产量明显增加。其中一个化合物经质谱和核磁共振鉴定为寡霉素D。结论:利用基因工程技术获得了一株产少霉素d的菌株Δspa7074。

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本刊更多论文

[Construction of SPA7074-deficient mutant of biocontrol strain Streptomyces pactum Act12 and characterization of its secondary metabolites].

Objective: To disrupt spa7074, which encodes a member of the TetR family transcriptional factors, in biocontrol strain Act12 and characterize the secondary metabolites in the mutant strain.

Methods: We disrupted the gene spa7074 by homologous recombination. The secondary metabolites of the mutant strain Δspa7074 and Act12 were detected by HPLC. The structure was analyzed by MS and NMR.

Results: Compared to the wild-type strain, the production of some unknown compounds in the mutant strain Δspa7074 increased obviously. We purified one of the compounds and identified as oligomycin D by MS and NMR analysis.

Conclusion: An oligomycin D-producing strain Δspa7074 was derived via genetic engineering.

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来源期刊

微生物学报

自引率

0.00%

发文量

7960

期刊介绍： Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.