[牛金黄色葡萄球菌FnBPA-A不同基因多态性重组物的免疫学特性比较]。

微生物学报 Pub Date : 2016-12-04

Caidie Wang, Yan Su, Lingling Su, Haina Wei, Baojiang Zhang

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引用次数: 0

摘要

目的:了解FnBPA-A基因多态性对金黄色葡萄球菌免疫生物学特性的影响。方法:分析新疆牛金黄色葡萄球菌FnBPA-A基因序列，构建8个不同基因多态性的真核重组体。用重组质粒免疫C57BL/6小鼠，收集小鼠血清。比较免疫小鼠的免疫保护水平。结果:GS801、GS819和GS856在同一支上;GW10-1、GW20-2、GY288、GY309属于同一分支;GY278在不同的分支上。在攻毒实验中，GW20-2、GS801、GS819、GS856和GY288表现出较好的保护作用。结论:FnBPA-A基因多态性可显著影响免疫小鼠的免疫保护作用。

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[Immunological characteristic comparison of different genetic polymorphism recombinant of FnBPA-A of bovine Staphylococcus aureus strain].

Objective: To understand the impact of genetic polymorphism of FnBPA-A on the immune biological characteristics of Staphylococcus aureus.

Methods: Sequence of FnBPA-A of Staphylococcus aureus isolated from bovine in Xinjiang was analyzed and 8 different genetic polymorphism eukaryotic recombinants of FnBPA-A were constructed. C57BL/6 mice were immunized with these recombinant plasmids and mice sera were collected. Level of the immune protection of immunized mice was compared.

Results: GS801, GS819 and GS856 were on the same branch; GW10-1, GW20-2, GY288 and GY309 belong to the same branch; GY278 was on a different branch. For the challenge experiment, GW20-2, GS801, GS819, GS856 and GY288 showed better protection.

Conclusion: The genetic polymorphism of FnBPA-A could significantly affect the immune protection of immunized mice.

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来源期刊

微生物学报

自引率

0.00%

发文量

7960

期刊介绍： Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.