戊型肝炎病毒:分离、繁殖和定量

Current Protocols in Microbiology Pub Date : 2018-02-22 DOI:10.1002/cpmc.50

Shaoli Lin, Liping Yang, Yan-Jin Zhang

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引用次数: 3

摘要

戊型肝炎病毒(HEV)主要引起人类急性肝病，并通过粪-口途径传播。孕妇感染戊型肝炎可导致严重后果，死亡率高达30%。感染人类的HEV毒株主要分为四种基因型。基因型1和2仅限于人感染，而基因型3和4是人畜共患。HEV基因3型(HEV-3)可引起急性和慢性肝脏疾病。几种细胞系(主要是肝细胞)已被开发用于HEV的繁殖和生物学研究。然而，在这些细胞系中生产HEV是次优和低效的。在这里，我们提出了HEV的分离、繁殖和定量方法。©2018 by John Wiley &儿子,Inc。

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Hepatitis E Virus: Isolation, Propagation, and Quantification

Hepatitis E virus (HEV) predominantly causes acute liver disease in humans and is transmitted via the fecal-oral route. HEV infection in pregnant women can result in grave consequences, with up to 30% fatality. The HEV strains infecting humans mainly belong to four genotypes. Genotypes 1 and 2 are restricted to human infection, while genotypes 3 and 4 are zoonotic. HEV genotype 3 (HEV-3) can cause both acute and chronic liver diseases. Several cell lines (mainly hepatocytes) have been developed for HEV propagation and biological study. However, HEV production in these cell lines is suboptimal and inefficient. Here, we present methods for the isolation, propagation, and quantification of HEV. © 2018 by John Wiley & Sons, Inc.

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来源期刊

Current Protocols in Microbiology Immunology and Microbiology-Parasitology

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期刊介绍： Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.