神经元肌囊虫的分子遗传操作

Current Protocols in Microbiology Pub Date : 2018-02-22 DOI:10.1002/cpmc.48

Daniel K. Howe, Michelle Yeargan, Landon Simpson, Sriveny Dangoudoubiyam

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引用次数: 3

摘要

神经性肌囊虫是重要的顶复合体门的成员，是马原生动物髓脑炎(EPM)的主要病因。此外，神经胞丝虫是肉囊虫属中研究得最好的物种，是最成功的寄生虫分类之一，因为几乎所有脊椎动物都可能被至少一种寄生虫感染。因此，对神经藻的科学研究将有助于海洋哺乳动物EPM和神经系统疾病的控制，同时也有助于我们对顶复体系统发育树上的一个重要分支的理解。这些协议描述的方法，扩大能力，以研究这一突出成员的顶复合体。©2018 by John Wiley &儿子,Inc。

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Molecular Genetic Manipulation of Sarcocystis neurona

Sarcocystis neurona is a member of the important phylum Apicomplexa and the primary cause of equine protozoal myeloencephalitis (EPM). Moreover, S. neurona is the best-studied species in the genus Sarcocystis, one of the most successful parasite taxa, as virtually all vertebrate animals may be infected by at least one species. Consequently, scientific investigation of S. neurona will aid in the control of EPM and neurologic disease in sea mammals, while also improving our understanding of a prominent branch on the apicomplexan phylogenetic tree. These protocols describe methods that expand the capabilities to study this prominent member of the Apicomplexa. © 2018 by John Wiley & Sons, Inc.

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Current Protocols in Microbiology Immunology and Microbiology-Parasitology

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期刊介绍： Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.