马来西亚不同产地苦豆(Parkia speciosa Hassk.)植物化学成分及生物活性的评价与比较。

Q1 Chemistry
Ali Ghasemzadeh, Hawa Z E Jaafar, Mohamad Fhaizal Mohamad Bukhori, Mohd Hafizad Rahmat, Asmah Rahmat
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引用次数: 36

摘要

背景:Parkia speciosa种子是一种常见的成分在马来菜与传统的兴趣,因为它的药用重要性和促进健康的植物化学物质的内容。本研究对采自马来西亚三个不同地区(霹雳州、森美兰州和柔佛州)的Parkia speciosa Hassk种子的植物化学成分和生物活性(抗氧化和抗菌活性)进行了评价。采用分光光度法测定植物化学成分(总黄酮和总酚),采用超高效液相色谱法鉴定单个类黄酮和酚酸。采用铁还原抗氧化电位(FRAP)测定和2,2-二苯基-1-苦味酰肼(DPPH)测定来评价其抗氧化活性。采用圆盘扩散法评价提取物对革兰氏阳性菌和革兰氏阴性菌的抑菌活性。结果:植物化学成分初步筛选表明,黄酮类化合物、生物碱、萜类化合物、酚类化合物等均为黄酮类化合物。从霹雳州收集的样本含有最高水平的植物化学成分,DPPH和FRAP活性最高,其次是森美兰州和柔佛。从鉴定的化合物中,槲皮素和没食子酸含量最多。从Perak地区收集的种子对革兰氏阳性和革兰氏阴性菌株都有很强的抗菌活性。金黄色葡萄球菌和枯草芽孢杆菌是最敏感的菌株。相关分析表明,黄酮类化合物与黄酮类化合物的抗氧化活性有关,而抗菌活性与没食子酸的含量密切相关。结论:生长在霹雳州的帕克兰种子具有最高的植物化学物质浓度和最高的生物活性。也可以建议食品工业使用该地区的种子来生产产品,这些产品将在不断扩大的功能食品市场上竞争。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment and comparison of phytochemical constituents and biological activities of bitter bean (Parkia speciosa Hassk.) collected from different locations in Malaysia.

Background: Parkia speciosa seeds are a common ingredient in Malay cuisine with traditional interest because of its medicinal importance and content of health-promoting phytochemicals. This study evaluated the phytochemical constituents and biological activities (antioxidant and antibacterial activities) of Parkia speciosa Hassk seeds collected from three different regions of Malaysia (Perak, Negeri Sembilan and Johor). Phytochemical constituents (total flavonoid and total phenolic) were measured using the spectrophotometric method, and individual flavonoids and phenolic acids were identified using ultra-high-performance liquid chromatography. Ferric reducing antioxidant potential (FRAP) assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay we used in order to evaluation of antioxidant activities. Disc diffusion method was employed for the evaluation of antibacterial activity of extracts against Gram-positive and Gram-negative bacterial strains.

Results: The primary screening of phytochemicals showed that P. speciosa seeds contain alkaloids, terpenoids, flavonoids, and phenolics. Samples collected from Perak contained the highest levels of the phytochemical constituents, with highest DPPH and FRAP activity followed by Negeri sembilan and Johor. From the identified compounds, quercetin and gallic acid were identified as the most abundant compounds. Seeds collected from the Perak location exhibited potent antibacterial activity, against both Gram-positive and Gram-negative bacteria strains. Staphylococcus aureus and Bacillus subtilis were recorded as the bacterial strains most sensitive to P. speciosa seed extracts. Correlation analysis showed that flavonoid compounds are responsible for the antioxidant activities of the P. speciosa seeds studied, while antibacterial activity showed a high correlation with the levels of gallic acid.

Conclusions: Parkia speciosa seed grown in Perak exhibit the highest concentrations of phytochemicals, as well as the highest biological activity. It may also be recommended for the food industry to use seeds from this area for their products, which are going to compete in the expanding functional food markets.

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来源期刊
Chemistry Central Journal
Chemistry Central Journal 化学-化学综合
CiteScore
4.40
自引率
0.00%
发文量
0
审稿时长
3.5 months
期刊介绍: BMC Chemistry is an open access, peer reviewed journal that considers all articles in the broad field of chemistry, including research on fundamental concepts, new developments and the application of chemical sciences to broad range of research fields, industry, and other disciplines. It provides an inclusive platform for the dissemination and discussion of chemistry to aid the advancement of all areas of research. Sections: -Analytical Chemistry -Organic Chemistry -Environmental and Energy Chemistry -Agricultural and Food Chemistry -Inorganic Chemistry -Medicinal Chemistry -Physical Chemistry -Materials and Macromolecular Chemistry -Green and Sustainable Chemistry
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