蛋白质与核酸鸟嘌呤四重结构相互作用的表征。

IF 1.3 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Journal of Nucleic Acids Pub Date : 2017-01-01 Epub Date: 2017-11-09 DOI:10.1155/2017/9675348
Ewan K S McRae, Evan P Booy, Gay Pauline Padilla-Meier, Sean A McKenna
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引用次数: 39

摘要

鸟嘌呤四联体(G4s)是核酸的四链二级结构,通过氮基之间的非规范氢键系统以及广泛的碱基堆叠或pi-pi相互作用来稳定。这些结构在基因组DNA或细胞RNA中的形成有可能在许多方面影响细胞生物学,包括端粒维持、转录、交替剪接和翻译。因此,G4s已成为治疗靶点,并且已经开发出几种可以结合此类结构的小分子化合物,但对于G4s如何与其天然蛋白质结合伙伴相互作用知之甚少。本文综述了蛋白质和小肽对G4s的识别,并比较了迄今为止观察到的识别模式。重点将放在通过G4/肽复合物的高分辨率晶体学和核磁共振结构以及结合特异性的生化研究获得的信息上。通过了解导致G4特异性与天然蛋白结合的分子特征,我们将更好地为靶向蛋白/G4相互作用的治疗目的做好准备。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

On Characterizing the Interactions between Proteins and Guanine Quadruplex Structures of Nucleic Acids.

On Characterizing the Interactions between Proteins and Guanine Quadruplex Structures of Nucleic Acids.

On Characterizing the Interactions between Proteins and Guanine Quadruplex Structures of Nucleic Acids.

On Characterizing the Interactions between Proteins and Guanine Quadruplex Structures of Nucleic Acids.

Guanine quadruplexes (G4s) are four-stranded secondary structures of nucleic acids which are stabilized by noncanonical hydrogen bonding systems between the nitrogenous bases as well as extensive base stacking, or pi-pi, interactions. Formation of these structures in either genomic DNA or cellular RNA has the potential to affect cell biology in many facets including telomere maintenance, transcription, alternate splicing, and translation. Consequently, G4s have become therapeutic targets and several small molecule compounds have been developed which can bind such structures, yet little is known about how G4s interact with their native protein binding partners. This review focuses on the recognition of G4s by proteins and small peptides, comparing the modes of recognition that have thus far been observed. Emphasis will be placed on the information that has been gained through high-resolution crystallographic and NMR structures of G4/peptide complexes as well as biochemical investigations of binding specificity. By understanding the molecular features that lead to specificity of G4 binding by native proteins, we will be better equipped to target protein/G4 interactions for therapeutic purposes.

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来源期刊
Journal of Nucleic Acids
Journal of Nucleic Acids BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
3.10
自引率
21.70%
发文量
5
审稿时长
12 weeks
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