尿道球菌（Aerococcus urinae）和血清球菌（Aerococcus sanguinicola）：使用盘式扩散（EUCAST）、Etest 和肉汤微量稀释技术检测 120 个分离株对六种抗菌剂的敏感性。

Q3 Immunology and Microbiology

Open Microbiology Journal Pub Date : 2017-09-21 eCollection Date: 2017-01-01 DOI:10.2174/1874285801711010160

Derya Carkaci, Xiaohui C Nielsen, Kurt Fuursted, Robert Skov, Ole Skovgaard, Emilio P Trallero, Reto Lienhard, Jenny Åhman, Erika Matuschek, Gunnar Kahlmeter, Jens J Christensen

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引用次数: 0

摘要

背景：尿道气球菌（Aerococcus urinae）和血尿气球菌（Aerococcus sanguinicola）是临床和微生物学实践中相对较新的新兴微生物。在全球范围内，这两种细菌都与尿路感染有关。据报道，菌血症/败血症和感染性心内膜炎的病例更为罕见。因此，治疗方案非常重要。最近，欧洲发布了关于药敏试验和解释标准的建议：在这项调查中，对 120 个尿囊炎甲菌和痢疾甲菌分离株进行了对六种抗菌药物的敏感性测试：青霉素、头孢他啶、美罗培南、万古霉素、利奈唑胺和利福平：采用了三种药敏试验方法：根据欧洲抗菌药物药敏试验委员会（EUCAST）的标准磁盘扩散法进行磁盘扩散，以及使用Etest和肉汤微量稀释法（BMD）进行MIC测定。所有测试均使用欧盟抗菌药物敏感性测试委员会（EUCAST）的快速微生物培养基进行：结果：本研究获得的数据是确定欧盟CAST断点的部分背景数据。Etest 和 BMD 得出的 MIC 值与磁盘扩散结果有很好的相关性：结论：所有分离菌株均对青霉素、头孢他啶、美罗培南、万古霉素、利奈唑胺和利福平这六种抗菌药物敏感。

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Aerococcus urinae and Aerococcus sanguinicola: Susceptibility Testing of 120 Isolates to Six Antimicrobial Agents Using Disk Diffusion (EUCAST), Etest, and Broth Microdilution Techniques.

Background: Aerococcus urinae and Aerococcus sanguinicola are relatively newcomers and emerging organisms in clinical and microbiological practice. Both species have worldwide been associated with urinary tract infections. More rarely cases of bacteremia/septicemia and infective endocarditis have been reported. Treatment options are therefore important. Just recently, European recommendations on susceptibility testing and interpretive criteria have been released.

Objective: In this investigation 120 A. urinae and A. sanguinicola isolates were tested for susceptibility to six antimicrobial agents: Penicillin, cefotaxime, meropenem, vancomycin, linezolid, and rifampicin.

Methods: Three susceptibility testing methods were used; disk diffusion according to The European Committee on Antimicrobial Susceptibility Testing (EUCAST) standardized disk diffusion methodology and MIC determination with Etest and broth microdilution (BMD). All testing was performed with EUCAST media for fastidious organisms.

Results: Data obtained in this study were part of the background data for establishing EUCAST breakpoints. MIC values obtained by Etest and BMD were well correlated with disk diffusion results.

Conclusion: All isolates were found susceptible to all six antimicrobial agents: penicillin, cefotaxime, meropenem, vancomycin, linezolid, and rifampicin.

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来源期刊

Open Microbiology Journal Immunology and Microbiology-Immunology and Microbiology (all)

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： The Open Microbiology Journal is a peer-reviewed open access journal which publishes research articles, reviews/mini-reviews, case studies, guest edited thematic issues and short communications/letters covering theoretical and practical aspects of Microbial systematics, evolutionary microbiology, immunology, virology, parasitology , bacteriology, mycology, phycology, protozoology, microbial ecology, molecular biology, microbial physiology, biochemistry, microbial pathogenesis, host-microbe interaction, systems microbiology, synthetic microbiology, bioinformatics. The Open Microbiology Journal , a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and freely available to researchers worldwide.