IL-1R8长异构体(TIR8/SIGIRR)的表征

IF 2.2 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

European cytokine network Pub Date : 2017-06-01 DOI:10.1684/ecn.2017.0395

Maria Giovanna Vilia, Marta Tocchetti, Eleonora Fonte, Ilenia Sana, Marta Muzio

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引用次数: 10

摘要

IL-1R8，也被称为SIGIRR或TIR8，是一种跨膜蛋白，属于IL-1受体家族。人类基因包括十个外显子，不同的剪接可以产生不同的异构体。在此，我们表征了IL-1R8的一个较长的同工异构体，该异构体在蛋白质的TIR结构域和c端部分之间含有一个帧内附加序列。IL-1R8长(IL-1R8L1) mRNA在不同细胞系中特异性表达和调控，其方式与经典亚型相似。IL-1R8L1的过表达导致相应蛋白的产生，该蛋白显示出与经典亚型相似的细胞定位模式。一种针对IL-1R8L1特异性肽的抗体，在不同的细胞系和组织中检测到这种新的异构体，这种蛋白质可能补充了经典IL-1R8的抗炎功能。

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Characterization of a long isoform of IL-1R8 (TIR8/SIGIRR).

IL-1R8, also known as SIGIRR or TIR8, is a trans-membrane protein belonging to the IL-1 receptor family. The human gene includes ten exons, and alternative splicing can result in different isoforms. We, herein, characterized a longer isoform of IL-1R8 containing an in-frame additional sequence between the TIR domain and the C-terminal portion of the protein. IL-1R8 Long (IL-1R8L1) mRNA was specifically expressed and regulated in distinct cell lines, in a manner similar to the classic isoform. Overexpression of IL-1R8L1 resulted in the production of a corresponding protein that showed a pattern of cell localization similar to the classic isoform. An antibody directed against an IL-1R8L1 specific peptide, detected this novel isoform in different cell lines and tissues where this protein may complement the anti-inflammatory functions of classic IL-1R8.

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来源期刊

European cytokine network 生物-免疫学

CiteScore

5.70

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： The journal that brings together all areas of work involving cytokines. European Cytokine Network is an electronic journal that publishes original articles and abstracts every quarter to provide an essential bridge between researchers and clinicians with an interest in this cutting-edge field. The journal has become a must-read for specialists in the field thanks to its swift publication and international circulation. The journal is referenced in several databases, including Medline, which is testament to its scientific quality.