基于pei的瞬时转染和慢病毒生产方法优化。

Q3 Biochemistry, Genetics and Molecular Biology

Current protocols in chemical biology Pub Date : 2017-09-14 DOI:10.1002/cpch.25

Shaozhe Yang, Xiaoling Zhou, Rongxiang Li, Xiuhong Fu, Pingnan Sun

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引用次数: 28

摘要

聚乙烯亚胺(PEI)是一种阳离子聚合物载体，与DNA形成复合物，然后将阴离子核酸携带到真核细胞中。基于pei的转染被广泛用于质粒DNA的瞬时转染。PEI转染的效率受到许多因素的影响，包括PEI/DNA复合物的制备方式、PEI与DNA的比例、DNA的浓度、PEI溶液的储存条件等等。考虑到主要的影响因素，我们对pei转染进行了优化，以提高其效率、重复性和一致性。本协议概述了使用PEI进行普通瞬时转染和慢病毒生产的步骤。©2017 by John Wiley & Sons, Inc。

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Optimized PEI-based Transfection Method for Transient Transfection and Lentiviral Production.

Polyethyleneimine (PEI), a cationic polymer vehicle, forms a complex with DNA which then can carry anionic nucleic acids into eukaryotic cells. PEI-based transfection is widely used for transient transfection of plasmid DNA. The efficiency of PEI-based transfection is affected by numerous factors, including the way the PEI/DNA complex is prepared, the ratio of PEI to DNA, the concentration of DNA, the storage conditions of PEI solutions, and more. Considering the major influencing factors, PEI-based transfection has been optimized to improve its efficiency, reproducibility, and consistency. This protocol outlines the steps for ordinary transient transfection and lentiviral production using PEI. © 2017 by John Wiley & Sons, Inc.

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Current protocols in chemical biology Biochemistry, Genetics and Molecular Biology-Biophysics

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