Lena Kröninger, Jacqueline Gottschling, Uwe Deppenmeier
{"title":"鲁米尼氏甲烷菌(Methanomassiliicoccus luminyensis)的生长特性和甲基转移酶编码基因的表达。","authors":"Lena Kröninger, Jacqueline Gottschling, Uwe Deppenmeier","doi":"10.1155/2017/2756573","DOIUrl":null,"url":null,"abstract":"<p><p>DNA sequence analysis of the human gut revealed the presence a seventh order of methanogens referred to as Methanomassiliicoccales. <i>Methanomassiliicoccus luminyensis</i> is the only member of this order that grows in pure culture. Here, we show that the organism has a doubling time of 1.8 d with methanol + H<sub>2</sub> and a growth yield of 2.4 g dry weight/mol CH<sub>4</sub>. <i>M. luminyensis</i> also uses methylamines + H<sub>2</sub> (monomethylamine, dimethylamine, and trimethylamine) with doubling times of 2.1-2.3 d. Similar cell yields were obtained with equimolar concentrations of methanol and methylamines with respect to their methyl group contents. The transcript levels of genes encoding proteins involved in substrate utilization indicated increased amounts of mRNA from the <i>mtaBC2</i> gene cluster in methanol-grown cells. When methylamines were used as substrates, mRNA of the <i>mtb/mtt</i> operon and of the <i>mtmBC1</i> cluster were found in high abundance. The transcript level of <i>mtaC2</i> was almost identical in methanol- and methylamine-grown cells, indicating that genes for methanol utilization were constitutively expressed in high amounts. The same observation was made with resting cells where methanol always yielded the highest CH<sub>4</sub> production rate independently from the growth substrate. Hence, <i>M. luminyensis</i> is adapted to habitats that provide methanol + H<sub>2</sub> as substrates.</p>","PeriodicalId":49105,"journal":{"name":"Archaea-An International Microbiological Journal","volume":"2017 ","pages":"2756573"},"PeriodicalIF":2.3000,"publicationDate":"2017-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5688252/pdf/","citationCount":"0","resultStr":"{\"title\":\"Growth Characteristics of <i>Methanomassiliicoccus luminyensis</i> and Expression of Methyltransferase Encoding Genes.\",\"authors\":\"Lena Kröninger, Jacqueline Gottschling, Uwe Deppenmeier\",\"doi\":\"10.1155/2017/2756573\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>DNA sequence analysis of the human gut revealed the presence a seventh order of methanogens referred to as Methanomassiliicoccales. <i>Methanomassiliicoccus luminyensis</i> is the only member of this order that grows in pure culture. Here, we show that the organism has a doubling time of 1.8 d with methanol + H<sub>2</sub> and a growth yield of 2.4 g dry weight/mol CH<sub>4</sub>. <i>M. luminyensis</i> also uses methylamines + H<sub>2</sub> (monomethylamine, dimethylamine, and trimethylamine) with doubling times of 2.1-2.3 d. Similar cell yields were obtained with equimolar concentrations of methanol and methylamines with respect to their methyl group contents. The transcript levels of genes encoding proteins involved in substrate utilization indicated increased amounts of mRNA from the <i>mtaBC2</i> gene cluster in methanol-grown cells. When methylamines were used as substrates, mRNA of the <i>mtb/mtt</i> operon and of the <i>mtmBC1</i> cluster were found in high abundance. The transcript level of <i>mtaC2</i> was almost identical in methanol- and methylamine-grown cells, indicating that genes for methanol utilization were constitutively expressed in high amounts. The same observation was made with resting cells where methanol always yielded the highest CH<sub>4</sub> production rate independently from the growth substrate. Hence, <i>M. luminyensis</i> is adapted to habitats that provide methanol + H<sub>2</sub> as substrates.</p>\",\"PeriodicalId\":49105,\"journal\":{\"name\":\"Archaea-An International Microbiological Journal\",\"volume\":\"2017 \",\"pages\":\"2756573\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2017-11-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5688252/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archaea-An International Microbiological Journal\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1155/2017/2756573\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2017/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archaea-An International Microbiological Journal","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1155/2017/2756573","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2017/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Growth Characteristics of Methanomassiliicoccus luminyensis and Expression of Methyltransferase Encoding Genes.
DNA sequence analysis of the human gut revealed the presence a seventh order of methanogens referred to as Methanomassiliicoccales. Methanomassiliicoccus luminyensis is the only member of this order that grows in pure culture. Here, we show that the organism has a doubling time of 1.8 d with methanol + H2 and a growth yield of 2.4 g dry weight/mol CH4. M. luminyensis also uses methylamines + H2 (monomethylamine, dimethylamine, and trimethylamine) with doubling times of 2.1-2.3 d. Similar cell yields were obtained with equimolar concentrations of methanol and methylamines with respect to their methyl group contents. The transcript levels of genes encoding proteins involved in substrate utilization indicated increased amounts of mRNA from the mtaBC2 gene cluster in methanol-grown cells. When methylamines were used as substrates, mRNA of the mtb/mtt operon and of the mtmBC1 cluster were found in high abundance. The transcript level of mtaC2 was almost identical in methanol- and methylamine-grown cells, indicating that genes for methanol utilization were constitutively expressed in high amounts. The same observation was made with resting cells where methanol always yielded the highest CH4 production rate independently from the growth substrate. Hence, M. luminyensis is adapted to habitats that provide methanol + H2 as substrates.
期刊介绍:
Archaea is a peer-reviewed, Open Access journal that publishes original research articles as well as review articles dealing with all aspects of archaea, including environmental adaptation, enzymology, genetics and genomics, metabolism, molecular biology, molecular ecology, phylogeny, and ultrastructure. Bioinformatics studies and biotechnological implications of archaea will be considered. Published since 2002, Archaea provides a unique venue for exchanging information about these extraordinary prokaryotes.