实验性矽肺。1 .气管内灌注石英对大鼠肺胶原代谢及形态学特征的急性影响。

The American Journal of Pathology Pub Date : 1982-05-01
K M Reiser, T W Hesterberg, W M Haschek, J A Last
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引用次数: 0

摘要

在第0天,大鼠气管内灌注50 mg石英(0.5 mu颗粒)。1周和2周后,通过组织学和各种生化测量对肺进行评估。定量测定蛋白质、脯氨酸和羟脯氨酸含量(作为肺总胶原蛋白的指标),测定肺胶原合成率和肺总蛋白生物合成率(用肺末体外评价)。测定新合成的I型/ III型胶原的比例,测定肺总胶原的比例。这些实验是在无慢性呼吸道疾病的大鼠和一种常规动物身上并行进行的。结论:1)气管内灌注50 mg石英后,肺结构和组成的变化最早可在1周(研究的最早时间点)检测到;2)前2周观察到的形态学变化与生化变化一致;3)无慢性呼吸道疾病Sprague-Dawley大鼠与常规Wistar大鼠对气管内灌注二氧化硅的反应基本无差异。两株大鼠均出现含硅肉芽肿,最终发展为硅结节,以及与间质性肺炎相关的肺泡脂蛋白沉积区。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Experimental silicosis. I. Acute effects of intratracheally instilled quartz on collagen metabolism and morphologic characteristics of rat lungs.

Rats were intratracheally instilled with 50 mg of silica as quartz (0.5-mu particles) at day 0. One and 2 weeks later, lungs were evaluated histologically and by a variety of biochemical measurements. Protein, proline, and hydroxyproline content (as an index of total lung collagen) were quantitated, as were the lung collagen synthesis rate and the total lung protein biosynthesis rate (evaluated with lung minces in vitro). The ratio of newly synthesized Type I/Type III collagen was determined, as was the same ratio for total lung collagen. These experiments were performed in parallel on chronic respiratory disease-free rats and in a strain of conventional animals. The authors conclude that 1) changes in lung structure and composition can be appreciated as early as 1 week, the earliest time point studied, after intratracheal instillation of 50 mg of quartz; 2) observed morphologic changes during the first 2 weeks are consistent with biochemical changes; 3) there are essentially no differences in the response of chronic respiratory disease-free Sprague-Dawley and conventional Wistar rats to intratracheally instilled silica. Both strains of rats developed silica-containing granulomas, which ultimately developed into silicotic nodules, as well as areas of alveolar lipoproteinosis associated with interstitial pneumonitis.

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