Yu D Ivanov, K A Malsagova, T O Pleshakova, I D Shumov, A L Kaysheva, V P Popov, O V Naumova, B I Fomin, D A Nasimov, A V Latyshev, A L Aseev, V Yu Tatur, N D Ivanova, G M Konovalova, A I Archakov
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引用次数: 0
摘要
提出了一种基于场效应纳米晶体管的纳米线生物传感器检测血清中肿瘤相关蛋白D-NFATc1的方法。在“绝缘体上硅”结构的基础上，制备了场效应纳米晶体管。为了检测目标蛋白的生物特异性，对NW表面进行了针对目标蛋白的适体修饰。使用3um - nw，可以获得稳定的源漏特性，并在漏源电流与栅极电压特性测量模式下，在浓度为2.5 x 1014 M时记录血清中的D-NFATc1。采用运行TURBO NBS软件的高速数据采集系统进行漏源电流与栅极电压特性测量模式的数据采集。
[Registration of the protein in the serum with a field-effect nanotransistor biosensor].
A method for detection of cancer-associated protein D-NFATc1 in serum using nanowire (NW) biosensor based on field-effect nanotransistor is developed. Field-effect nanotransistor was fabricated on the basis of «silicon-on-insulator» structures. For the biospecific detection of target protein, the NW surface was modified with aptamers against the target protein. Using the 3 um-NW enabled to obtain stable source-drain characteristics and to register D-NFATc1 in serum at concentration of 2.5 x 1014 M in the mode of drain-source current vs. gate voltage characteristics measurements. Data collection in the mode of drain-source current vs. gate voltage characteristics measurements was carried out with the use of high-speed data collection system running TURBO NBS software.
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