Mehdi Abbasi, Majid BaseriSalehi, Nima Bahador, Morovat Taherikalani
{"title":"伊朗伊拉姆一家大医院分离的不同SCCmec和Pulsotypes金黄色葡萄球菌的抗生素耐药模式和毒力决定因素","authors":"Mehdi Abbasi, Majid BaseriSalehi, Nima Bahador, Morovat Taherikalani","doi":"10.2174/1874285801711010211","DOIUrl":null,"url":null,"abstract":"<p><strong>Aims & objectives: </strong>The aim of this study <b>is</b> to evaluate genetic relatedness, antibiotic resistance pattern, and virulence characteristics of different types of <i>S. aureus</i> isolated from air, surfaces, staff, and patients in a Public hospital in Ilam.</p><p><strong>Methods & materials: </strong>A total of 88 of 140 staphylococci identified as <i>S. aureus</i> by conventional and molecular methods were used in this study. Isolate samples were obtained from surfaces, staff, patients, and hospital indoor air. The sampling from staff and surfaces was done through using swab and air by standard pump. Antimicrobial susceptibility testing and presence different resistant and virulence determinants was assessed. Isolates were then typed by pulsed-field gel electrophoresis (PFGE) and SCC<i>mec</i> typing methods.</p><p><strong>Results: </strong>Out of 88isolates, 36 of them (40.9%) were MRSA. Among MRSA isolates, the range of resistance to antibiotic was 0% in vancomycin to 83.3% in gentamycin. The most prevalent resistant genes among gentamicin resistant <i>S. aureus</i> were <i>acc (6')/aph (2\")Ia</i> and <i>aph(3\")IIIa</i>. The most common erythromycin resistant gene was <i>erm</i>C. Surprisingly, SCC<i>mec</i> types I (30.5%), II (25%)were highly distributed. PFGE analysis showed 33 different pulsotypes.</p><p><strong>Conclusion: </strong>This study confirms that different isolates of MSSA and MRSA circulate in Ilam which differ in antimicrobial susceptibility, content of resistance, and virulence determinants.</p>","PeriodicalId":38953,"journal":{"name":"Open Microbiology Journal","volume":"11 ","pages":"211-223"},"PeriodicalIF":0.0000,"publicationDate":"2017-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5688384/pdf/","citationCount":"10","resultStr":"{\"title\":\"Antibiotic Resistance Patterns and Virulence Determinants of Different SCCmec and Pulsotypes of <i>Staphylococcus Aureus</i> Isolated from a Major Hospital in Ilam, Iran.\",\"authors\":\"Mehdi Abbasi, Majid BaseriSalehi, Nima Bahador, Morovat Taherikalani\",\"doi\":\"10.2174/1874285801711010211\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aims & objectives: </strong>The aim of this study <b>is</b> to evaluate genetic relatedness, antibiotic resistance pattern, and virulence characteristics of different types of <i>S. aureus</i> isolated from air, surfaces, staff, and patients in a Public hospital in Ilam.</p><p><strong>Methods & materials: </strong>A total of 88 of 140 staphylococci identified as <i>S. aureus</i> by conventional and molecular methods were used in this study. Isolate samples were obtained from surfaces, staff, patients, and hospital indoor air. The sampling from staff and surfaces was done through using swab and air by standard pump. Antimicrobial susceptibility testing and presence different resistant and virulence determinants was assessed. Isolates were then typed by pulsed-field gel electrophoresis (PFGE) and SCC<i>mec</i> typing methods.</p><p><strong>Results: </strong>Out of 88isolates, 36 of them (40.9%) were MRSA. Among MRSA isolates, the range of resistance to antibiotic was 0% in vancomycin to 83.3% in gentamycin. The most prevalent resistant genes among gentamicin resistant <i>S. aureus</i> were <i>acc (6')/aph (2\\\")Ia</i> and <i>aph(3\\\")IIIa</i>. The most common erythromycin resistant gene was <i>erm</i>C. Surprisingly, SCC<i>mec</i> types I (30.5%), II (25%)were highly distributed. PFGE analysis showed 33 different pulsotypes.</p><p><strong>Conclusion: </strong>This study confirms that different isolates of MSSA and MRSA circulate in Ilam which differ in antimicrobial susceptibility, content of resistance, and virulence determinants.</p>\",\"PeriodicalId\":38953,\"journal\":{\"name\":\"Open Microbiology Journal\",\"volume\":\"11 \",\"pages\":\"211-223\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-10-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5688384/pdf/\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Open Microbiology Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/1874285801711010211\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2017/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"Immunology and Microbiology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Microbiology Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874285801711010211","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2017/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"Immunology and Microbiology","Score":null,"Total":0}
Antibiotic Resistance Patterns and Virulence Determinants of Different SCCmec and Pulsotypes of Staphylococcus Aureus Isolated from a Major Hospital in Ilam, Iran.
Aims & objectives: The aim of this study is to evaluate genetic relatedness, antibiotic resistance pattern, and virulence characteristics of different types of S. aureus isolated from air, surfaces, staff, and patients in a Public hospital in Ilam.
Methods & materials: A total of 88 of 140 staphylococci identified as S. aureus by conventional and molecular methods were used in this study. Isolate samples were obtained from surfaces, staff, patients, and hospital indoor air. The sampling from staff and surfaces was done through using swab and air by standard pump. Antimicrobial susceptibility testing and presence different resistant and virulence determinants was assessed. Isolates were then typed by pulsed-field gel electrophoresis (PFGE) and SCCmec typing methods.
Results: Out of 88isolates, 36 of them (40.9%) were MRSA. Among MRSA isolates, the range of resistance to antibiotic was 0% in vancomycin to 83.3% in gentamycin. The most prevalent resistant genes among gentamicin resistant S. aureus were acc (6')/aph (2")Ia and aph(3")IIIa. The most common erythromycin resistant gene was ermC. Surprisingly, SCCmec types I (30.5%), II (25%)were highly distributed. PFGE analysis showed 33 different pulsotypes.
Conclusion: This study confirms that different isolates of MSSA and MRSA circulate in Ilam which differ in antimicrobial susceptibility, content of resistance, and virulence determinants.
期刊介绍：
The Open Microbiology Journal is a peer-reviewed open access journal which publishes research articles, reviews/mini-reviews, case studies, guest edited thematic issues and short communications/letters covering theoretical and practical aspects of Microbial systematics, evolutionary microbiology, immunology, virology, parasitology , bacteriology, mycology, phycology, protozoology, microbial ecology, molecular biology, microbial physiology, biochemistry, microbial pathogenesis, host-microbe interaction, systems microbiology, synthetic microbiology, bioinformatics. The Open Microbiology Journal , a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and freely available to researchers worldwide.
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