不同浓度α-龙葵碱对大鼠骨髓间充质干细胞影响的可靠、可重复模型

Bone Marrow Research Pub Date : 2017-01-01 Epub Date: 2017-10-22 DOI:10.1155/2017/2170306
Adriana Ordóñez-Vásquez, Lorenza Jaramillo-Gómez, Camilo Duran-Correa, Erandi Escamilla-García, Myriam Angélica De la Garza-Ramos, Fernando Suárez-Obando
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引用次数: 3

摘要

Αlpha-solanine (α-茄碱)是马铃薯(Solanum tuberosum)中的一种糖生物碱。由于其毒性和潜在的致畸作用,包括中枢神经系统异常,如脑畸形、脑膨出和眼失。以不同类型的细胞培养为实验模型,研究α-龙葵碱对细胞生理的影响。α-龙葵碱对间充质干细胞的影响尚不清楚。本研究旨在建立一种可靠、可重复的模型,用于评估小鼠骨髓间充质干细胞(MSCs)暴露于不同浓度α-龙葵碱24小时后的结构变化。结果表明,非致死浓度α-龙葵碱(2-6 μM)改变了细胞形态,包括核仁数量增加,表明蛋白质合成增加,针状体形成。此外,α-龙葵碱处理减少了培养中贴壁细胞数量和菌落形成。MSCs的免疫表型表征和染色被提议作为一种可重复的方法,允许描述暴露于糖生物碱α-龙葵碱的细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A Reliable and Reproducible Model for Assessing the Effect of Different Concentrations of <i>α</i>-Solanine on Rat Bone Marrow Mesenchymal Stem Cells.

A Reliable and Reproducible Model for Assessing the Effect of Different Concentrations of <i>α</i>-Solanine on Rat Bone Marrow Mesenchymal Stem Cells.

A Reliable and Reproducible Model for Assessing the Effect of Different Concentrations of <i>α</i>-Solanine on Rat Bone Marrow Mesenchymal Stem Cells.

A Reliable and Reproducible Model for Assessing the Effect of Different Concentrations of α-Solanine on Rat Bone Marrow Mesenchymal Stem Cells.

Αlpha-solanine (α-solanine) is a glycoalkaloid present in potato (Solanum tuberosum). It has been of particular interest because of its toxicity and potential teratogenic effects that include abnormalities of the central nervous system, such as exencephaly, encephalocele, and anophthalmia. Various types of cell culture have been used as experimental models to determine the effect of α-solanine on cell physiology. The morphological changes in the mesenchymal stem cell upon exposure to α-solanine have not been established. This study aimed to describe a reliable and reproducible model for assessing the structural changes induced by exposure of mouse bone marrow mesenchymal stem cells (MSCs) to different concentrations of α-solanine for 24 h. The results demonstrate that nonlethal concentrations of α-solanine (2-6 μM) changed the morphology of the cells, including an increase in the number of nucleoli, suggesting elevated protein synthesis, and the formation of spicules. In addition, treatment with α-solanine reduced the number of adherent cells and the formation of colonies in culture. Immunophenotypic characterization and staining of MSCs are proposed as a reproducible method that allows description of cells exposed to the glycoalkaloid, α-solanine.

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