T细胞嵌合抗原受体的遗传修饰:实验室手册。

Q1 Immunology and Microbiology

Human Gene Therapy Methods Pub Date : 2017-12-01 Epub Date: 2017-07-24 DOI:10.1089/hgtb.2017.083

Viktória Golumba-Nagy, Johannes Kuehle, Hinrich Abken

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引用次数: 14

摘要

嵌合抗原受体(CAR)基因修饰的重定向T细胞在早期试验中诱导了难治性白血病/淋巴瘤的显著缓解，吸引了将CAR - T细胞用于包括实体癌和非恶性疾病在内的各种其他应用的兴趣。然而，广泛的临床前探索需要高效和稳健的血液T细胞遗传修饰程序;这同样适用于重组T细胞受体的工程。我们在一步一步的方案中提出了实验室程序，通过γ-逆转录或慢病毒转导来设计人类和小鼠T细胞，以进行进一步的临床前测试。

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Genetic Modification of T Cells with Chimeric Antigen Receptors: A Laboratory Manual.

Redirected T cells genetically modified with a chimeric antigen receptor (CAR) have induced spectacular remissions of refractory leukemia/lymphoma in early phase trials, attracting interest to use CAR T cells in a variety of other applications including solid cancer and nonmalignant diseases. However, extensive preclinical explorations demand highly effective and robust procedures for the genetic modification of blood T cells; the same applies for engineering with a recombinant T cell receptor. We present laboratory procedures in a step-by-step protocol to engineer human and mouse T cells with a CAR by γ-retro- or lentiviral transduction for further preclinical testing.

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来源期刊

Human Gene Therapy Methods BIOTECHNOLOGY & APPLIED MICROBIOLOGY-GENETICS & HEREDITY

CiteScore

5.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Human Gene Therapy is the premier, multidisciplinary journal covering all aspects of gene therapy. The Journal publishes in-depth coverage of DNA, RNA, and cell therapies by delivering the latest breakthroughs in research and technologies. Human Gene Therapy provides a central forum for scientific and clinical information, including ethical, legal, regulatory, social, and commercial issues, which enables the advancement and progress of therapeutic procedures leading to improved patient outcomes, and ultimately, to curing diseases. The Journal is divided into three parts. Human Gene Therapy, the flagship, is published 12 times per year. HGT Methods, a bimonthly journal, focuses on the applications of gene therapy to product testing and development. HGT Clinical Development, a quarterly journal, serves as a venue for publishing data relevant to the regulatory review and commercial development of cell and gene therapy products.