Helen Mier-Giraldo, Luis Eduardo Díaz-Barrera, Lucy Gabriela Delgado-Murcia, Manuel Fernando Valero-Valdivieso, Gabriela Cáez-Ramírez
{"title":"紫浆果提取物对宫颈癌细胞(HeLa)和成纤维细胞(L929)的细胞毒性和免疫调节活性。","authors":"Helen Mier-Giraldo, Luis Eduardo Díaz-Barrera, Lucy Gabriela Delgado-Murcia, Manuel Fernando Valero-Valdivieso, Gabriela Cáez-Ramírez","doi":"10.1177/2156587217718751","DOIUrl":null,"url":null,"abstract":"<p><p>It was purposed to evaluate the biological potential of ethanol and isopropanol crude extracts of ripe Physalis peruviana fruits. Cytotoxic and immunomodulatory effects of the expression of interleukin-6, interleukin-8, and monocyte chemoattractant protein-1 (MCP-1) were evaluated on human cervical cancer (HeLa) and murine fibroblast (L929) cells. The composition was evaluated by high-performance liquid chromatography diode-array detection and high-performance liquid chromatography ultraviolet/visible detection. The presence of ursolic acid and rosmarinic acid was found in both solvents. However, gallic acid, quercetin, and epicatechin were higher in isopropanol extracts ( P < .05). The results indicated a relationship among the total polyphenol content, antioxidant activity, and cytotoxic activity that was dependent on the solvent used. Isopropanol extracts presented a half-maximal inhibition concentration value (IC<sub>50</sub>) of 60.48 ± 3.8 μg/mL for HeLa cells and 66.62 ± 2.67 μg/mL for L929 fibroblasts. The extracts reduced the release of interleukin-6, interleukin-8, and MCP-1 in a dose-dependent manner. Extracts showed anticancer and immunomodulatory potential for new complementary pharmaceutical products development.</p>","PeriodicalId":15804,"journal":{"name":"Journal of Evidence-Based Complementary & Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/2156587217718751","citationCount":"18","resultStr":"{\"title\":\"Cytotoxic and Immunomodulatory Potential Activity of Physalis peruviana Fruit Extracts on Cervical Cancer (HeLa) and Fibroblast (L929) Cells.\",\"authors\":\"Helen Mier-Giraldo, Luis Eduardo Díaz-Barrera, Lucy Gabriela Delgado-Murcia, Manuel Fernando Valero-Valdivieso, Gabriela Cáez-Ramírez\",\"doi\":\"10.1177/2156587217718751\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>It was purposed to evaluate the biological potential of ethanol and isopropanol crude extracts of ripe Physalis peruviana fruits. Cytotoxic and immunomodulatory effects of the expression of interleukin-6, interleukin-8, and monocyte chemoattractant protein-1 (MCP-1) were evaluated on human cervical cancer (HeLa) and murine fibroblast (L929) cells. The composition was evaluated by high-performance liquid chromatography diode-array detection and high-performance liquid chromatography ultraviolet/visible detection. The presence of ursolic acid and rosmarinic acid was found in both solvents. However, gallic acid, quercetin, and epicatechin were higher in isopropanol extracts ( P < .05). The results indicated a relationship among the total polyphenol content, antioxidant activity, and cytotoxic activity that was dependent on the solvent used. Isopropanol extracts presented a half-maximal inhibition concentration value (IC<sub>50</sub>) of 60.48 ± 3.8 μg/mL for HeLa cells and 66.62 ± 2.67 μg/mL for L929 fibroblasts. The extracts reduced the release of interleukin-6, interleukin-8, and MCP-1 in a dose-dependent manner. Extracts showed anticancer and immunomodulatory potential for new complementary pharmaceutical products development.</p>\",\"PeriodicalId\":15804,\"journal\":{\"name\":\"Journal of Evidence-Based Complementary & Alternative Medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1177/2156587217718751\",\"citationCount\":\"18\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Evidence-Based Complementary & Alternative Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1177/2156587217718751\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2017/7/18 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Evidence-Based Complementary & Alternative Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/2156587217718751","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2017/7/18 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
Cytotoxic and Immunomodulatory Potential Activity of Physalis peruviana Fruit Extracts on Cervical Cancer (HeLa) and Fibroblast (L929) Cells.
It was purposed to evaluate the biological potential of ethanol and isopropanol crude extracts of ripe Physalis peruviana fruits. Cytotoxic and immunomodulatory effects of the expression of interleukin-6, interleukin-8, and monocyte chemoattractant protein-1 (MCP-1) were evaluated on human cervical cancer (HeLa) and murine fibroblast (L929) cells. The composition was evaluated by high-performance liquid chromatography diode-array detection and high-performance liquid chromatography ultraviolet/visible detection. The presence of ursolic acid and rosmarinic acid was found in both solvents. However, gallic acid, quercetin, and epicatechin were higher in isopropanol extracts ( P < .05). The results indicated a relationship among the total polyphenol content, antioxidant activity, and cytotoxic activity that was dependent on the solvent used. Isopropanol extracts presented a half-maximal inhibition concentration value (IC50) of 60.48 ± 3.8 μg/mL for HeLa cells and 66.62 ± 2.67 μg/mL for L929 fibroblasts. The extracts reduced the release of interleukin-6, interleukin-8, and MCP-1 in a dose-dependent manner. Extracts showed anticancer and immunomodulatory potential for new complementary pharmaceutical products development.
期刊介绍：
The Journal of Evidence-Based Integrative Medicine ((JEBIM)), published previously as the Journal of Evidence-Based Complementary and Alternative Medicine (JEBCAM) and also as Complementary Health Practice Review (CHPR). The Journal of Evidence-Based Integrative Medicine (JEBIM) is an interdisciplinary, peer-reviewed, open access, biomedical journal whose aim is to create a global platform for hypothesis-driven and evidence-based research in all fields of integrative medicine. The journal’s objective is to publish papers which impart scientific validity to Integrative Medicine methods that are indispensable and inevitable in today’s world. All papers will be peer reviewed by experts in their respective fields, and papers will be accepted based on their scientific merit. It is the goal of the Journal to help remove the “myth” and provide scientific rationale for the various methodologies and theories of Integrative Medicine. All submissions will be reviewed based on their scientific merit and only papers with sound study design, valid statistical analyses and logical conclusions will be accepted. Topics include, but are not limited to: Traditional Eastern and Western medicine Nutrition therapy and supplementation Massage Therapy Non-traditional treatments Preventative medicine Integrative health and medicine Mindfulness Yoga.
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