运动和寒冷暴露对骨骼肌和白色脂肪组织线粒体生物发生的影响。

Nana Chung, Jonghoon Park, Kiwon Lim
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引用次数: 28

摘要

目的:本研究的目的是确定运动或/和冷暴露是否调节小鼠比目鱼和腹股沟脂肪组织中线粒体生物发生相关基因的表达。方法:将40只5周龄ICR雄性小鼠分为四组:热中性-未训练组(室温23±1℃,n=10)、冷水浸泡组(24±1℃,n=10)、中性运动组(34±1℃,n=10)和低温运动组(24±1℃,n=10)。小鼠进行游泳运动(30 ~ 60分钟,5次),连续8周。8周后,我们证实了比目鱼肌和腹股沟脂肪组织中过氧化物酶体增殖物激活受体γ辅助激活因子-1α (PGC-1α)、核呼吸因子1 (NRF1)和线粒体转录因子A (Tfam)线粒体生物发生相关基因的表达变化,以及比目鱼肌中相关蛋白的表达。结果:冷暴露(p = 0.006)和运动(p = 0.05)后,PGC-1α在比目鱼肌中的表达显著升高。运动和寒冷暴露之间也有显著的相互作用(p = 0.005)。只有运动对NRF1相对表达有显著影响(p=0.001)。冷暴露和相互作用均无显著影响(p = 0.1222和p = 0.875)。运动对Tfam的相对表达没有显著影响。在腹股沟脂肪组织中,各组PGC-1α的相对表达量均无显著变化。NRF1的表达在运动(p = 0.01)和冷暴露(p = 0.011)后均有显著变化。运动和寒冷暴露之间也有显著的相互作用(p = 0.000)。运动对Tfam mRNA表达有显著影响(p=0.000),运动与冷暴露之间存在交互作用(p=0.001)。只有温度显著影响PGC-1α蛋白水平(p=0.045)。运动和相互作用均不显著(p = 0.397和p = 0.292)。NRF1蛋白水平在任何实验处理中都没有显示出显著的影响。Tfam蛋白水平在运动组显示出显著影响(p=0.012),但冷暴露和相互作用的影响均不显著(p= 0.085和p=0.374)。结论:运动和冷暴露可增加比目鱼肌线粒体生物发生相关基因的表达。只有冷暴露对PGC-1α蛋白表达有显著影响,只有运动对Tfam蛋白表达有显著影响。在腹股沟脂肪组织中,运动和冷暴露在线粒体生物发生相关基因的表达中存在相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The effects of exercise and cold exposure on mitochondrial biogenesis in skeletal muscle and white adipose tissue.

The effects of exercise and cold exposure on mitochondrial biogenesis in skeletal muscle and white adipose tissue.

The effects of exercise and cold exposure on mitochondrial biogenesis in skeletal muscle and white adipose tissue.

The effects of exercise and cold exposure on mitochondrial biogenesis in skeletal muscle and white adipose tissue.

Purpose: The purpose of this study was to determine whether exercise or/and cold exposure regulate mitochondria biogenesis-related gene expression in soleus and inguinal adipose tissue of mice.

Methods: Forty ICR 5-week old male mice were divided into four groups: thermoneutrality-untrained (23 ± 1 °C in room temperature, n=10), cold-water immersion (24 ± 1 °C, n=10), exercise in neutral temperature (34 ± 1 °C, n=10), and exercise in cold temperature (24 ± 1 °C, n=10). The mice performed swimming exercise (30 min to 60 min, 5 times) for 8 weeks. After 8 weeks, we confirmed mitochondrial biogenesis-related gene expression changes for peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α), nuclear respiratory factors 1 (NRF1), and mitochondrial transcription factor A (Tfam) in soleus muscle and inguinal adipose tissue, and the related protein expression in soleus muscle.

Results: In soleus muscle, PGC-1α expression significantly increased in response to cold exposure (p = 0.006) and exercise (p = 0.05). There was also significant interaction between exercise and cold exposure (p = 0.005). Only exercise had a significant effect on NRF1 relative expression (p=0.001). Neither cold exposure nor the interaction showed significant effects (p = 0.1222 and p = 0.875, respectively). Relative Tfam expression did not show any significant effect from exercise. In inguinal adipose tissue, relative PGC-1α expression did not significantly change in any group. NRF1 expression showed a significant change from exercise (p = 0.01) and cold exposure (p = 0.011). There was also a significant interaction between exercise and cold exposure (p = 0.000). Tfam mRNA expression showed a significant effect from exercise (p=0.000) and an interaction between exercise and cold exposure (p=0.001). Only temperature significantly affected PGC-1α protein levels (p=0.045). Neither exercise nor the interaction were significant (p = 0.397 and p = 0.292, respectively). NRF1 protein levels did not show a significant effect in any experimental treatments. Tfam protein levels showed a significant effect in the exercise group (p=0.012), but effects of neither cold exposure nor the interaction were significant (p = 0.085 and p=0.374, respectively).

Conclusion: Exercise and cold exposure promoted increased expression of mitochondrial biogenesis- related genes in soleus muscle. Only cold exposure had a significant effect on PGC-1α protein expression and only exercise had a significant effect on Tfam protein expression. In inguinal adipose tissue, there was interaction between exercise and cold exposure in expression of mitochondrial biogenesis-related genes.

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