哥伦比亚加勒比海地区红海龟巢的从头转录组组装

Javier Hernández-Fernández , Andrés Pinzón , Leonardo Mariño-Ramírez
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引用次数: 6

摘要

红海龟广泛分布于热带和亚热带纬度的海洋。由于人为和自然因素,这种龟是一种濒临灭绝的物种,它们的数量减少了。在这项研究中,我们对血液中表达的基因进行了RNA测序和从头组装。原始FASTQ文件已存储在NCBI的SRA数据库中,登录号为SRX2629512。总共获得5.4 Gb的原始序列数据,对应于48,257,019个原始读取。利用Trinity流水线进行从头组装,共鉴定出64930个雌性红海龟转录组,N50值为1131 bp。获得的转录组数据将为进一步研究该物种的生理、生物化学和进化提供有用的数据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
De novo transcriptome assembly of loggerhead sea turtle nesting of the Colombian Caribbean

Loggerhead sea turtle Caretta caretta is widely distributed in the oceans of tropical and subtropical latitude. This turtle is an endangered species due to anthropic and natural factors that have decreased their population levels. In this study, RNA sequencing and de-novo assembly of genes expressed in blood were performed. The raw FASTQ files have been deposited on NCBI's SRA database with accession number SRX2629512. A total of 5.4 Gb raw sequence data were obtained, corresponding to 48,257,019 raw reads. Trinity pipeline was used to perform a de-novo assembly, we were able to identify 64,930 transcripts for female loggerhead turtle transcriptome with an N50 of 1131 bp. The obtained transcriptome data will be useful for further studies of the physiology, biochemistry and evolution in this species.

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