产油酵母红酵母菌株CGMCC 2.1609的基因组序列

Christine Sambles, Sabine Middelhaufe, Darren Soanes, Dagmara Kolak, Thomas Lux, Karen Moore, Petra Matoušková, David Parker, Rob Lee, John Love, Stephen J. Aves
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引用次数: 13

摘要

大多数真核生物产油物种是酵母,其中担子菌红酵母(Rhodosporidium) toruloides (puccininiomycotina)在限氮培养基中生长时已知能产生大量脂质,并具有生产生物柴油的潜力。利用Roche 454和Illumina杂交技术对产油红酵母CGMCC 2.1609株基因组进行测序,获得13 ×覆盖。使用MIRA进行从头组装,并使用MAQ和BAMBUS进行支架。测序组装得到365个支架,总基因组大小为33.4 Mb。该菌株的全基因组序列保存在GenBank中,登录号为LKER00000000。该注释可在Figshare上获得(doi:10.6084/m9.figshare.4754251)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome sequence of the oleaginous yeast Rhodotorula toruloides strain CGMCC 2.1609

Most eukaryotic oleaginous species are yeasts and among them the basidiomycete red yeast, Rhodotorula (Rhodosporidium) toruloides (Pucciniomycotina) is known to produce high quantities of lipids when grown in nitrogen-limiting media, and has potential for biodiesel production. The genome of the CGMCC 2.1609 strain of this oleaginous red yeast was sequenced using a hybrid of Roche 454 and Illumina technology generating 13 × coverage. The de novo assembly was carried out using MIRA and scaffolded using MAQ and BAMBUS. The sequencing and assembly resulted in 365 scaffolds with total genome size of 33.4 Mb. The complete genome sequence of this strain was deposited in GenBank and the accession number is LKER00000000. The annotation is available on Figshare (doi:10.6084/m9.figshare.4754251).

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