Synechocystis sp. PCC 6803自养和光养生长过程中双组分信号转导系统在调节中心碳水化合物和能量代谢中的串音

IF 1.4
Guangsheng Pei, Xiangfeng Niu, Yuqing Zhou, Lei Chen, Weiwen Zhang
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引用次数: 5

摘要

单细胞模式蓝藻Synechocystis sp. PCC 6803由于其光合机制而作为一种可持续能源受到了广泛关注。然而,由于蓝藻的多样性和功能复杂性,双组分信号转导系统(TCSTSs)在调节蓝藻中央碳水化合物和能量代谢中的作用仍然知之甚少。在这项研究中,通过比较44个TCSTSs反应调控因子(RR)的敲除突变体在聚胞菌中的生长情况,在自动或光养条件下发现了几个表现出不同生长模式的RR突变体。然而,尽管剩余的RR突变体没有观察到生长差异,但基于液相色谱-质谱的代谢组学分析表明,TCSTSs在调节聚囊藻中心碳水化合物和能量代谢方面存在广泛的串串,而大多数TCSTSs在不同的营养类型或生长阶段表现出不同的模式。此外,通过对进化关系和代谢组学特征的综合分析,发现了一些具有高度功能趋同的同源RRs对,这表明Synechocystis TCSTSs在进化过程中可能具有保守功能。该研究为了解TCSTSs在光合蓝藻中的作用奠定了重要基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Crosstalk of two-component signal transduction systems in regulating central carbohydrate and energy metabolism during autotrophic and photomixotrophic growth of Synechocystis sp. PCC 6803.

Unicellular model cyanobacterium Synechocystis sp. PCC 6803 has received considerable attention as a sustainable energy resource because of its photosynthetic machinery. However, two-component signal transduction systems (TCSTSs) in regulating central carbohydrate and energy metabolism of cyanobacteria are still poorly understood due to their diversity and functional complication. In this study, by comparing the growth of knockout mutants of 44 response regulators (RRs) of TCSTSs in Synechocystis, several RR mutants demonstrating differential growth patterns were identified under auto- or photomixotrophic conditions. However, in spite of no growth difference observed for the remaining RR mutants, liquid chromatography-mass spectrometry based metabolomic profile analysis showed that a widespread crosstalk of TCSTSs in regulating central carbohydrate and energy metabolism of Synechocystis was identified, while most of them showed diverse patterns during different trophic types or growth stages. Furthermore, an integrative analysis between evolutionary relationships and metabolomic profiles revealed some pairs of paralogous RRs with highly functional convergence, suggesting the possible conserved functions of Synechocystis TCSTSs during evolution. This study laid an important basis for understanding the function of TCSTSs in photosynthetic cyanobacteria.

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