自体骨髓抽吸联合人甲状旁腺激素1-34治疗家兔骨坏死模型的可行性及疗效。

Bone Marrow Research Pub Date : 2017-01-01 Epub Date: 2017-03-13 DOI:10.1155/2017/2484689
Takeshi Makihara, Tomokazu Yoshioka, Hisashi Sugaya, Katsuya Aoto, Hiroshi Wada, Kenta Uemura, Kenta Tanaka, Hiroshi Akaogi, Masashi Yamazaki, Hajime Mishima
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引用次数: 3

摘要

目前还没有研究检测含有间充质干细胞(MSCs)的骨髓抽吸液(BMA)联合人甲状旁腺激素1-34 (hPTH1-34)的移植。因此,我们在骨坏死模型中评估自体BMA移植联合hPHT1-34给药的可行性和疗效。采用液氮对兔跖骨进行坏死处理,术后给予BMA移植或生理盐水注射,再给予hPTH1-34 (30 μg/kg)或生理盐水,每周3次(每组n = 3-4次)。治疗开始后12周对家兔实施安乐死。没有观察到全身不良反应或局部肿瘤病变。重要的是,BMA + hPTH1-34组的兔骨体积和新骨组织学评分最高。这些数据至少在实验期间证实了BMA联合hPTH1-34移植的可行性。观察到的效果可能是通过刺激MSC分化成骨细胞与hpth1 -34介导的成骨细胞凋亡抑制的协同作用来解释的。这些结果表明BMA移植联合hPTH1-34治疗骨坏死有很大的潜力。需要更长期的实验来证实这种治疗策略的安全性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Feasibility and Efficacy of Autologous Bone Marrow Aspirate Transplantation Combined with Human Parathyroid Hormone 1-34 Administration to Treat Osteonecrosis in a Rabbit Model.

Feasibility and Efficacy of Autologous Bone Marrow Aspirate Transplantation Combined with Human Parathyroid Hormone 1-34 Administration to Treat Osteonecrosis in a Rabbit Model.

Feasibility and Efficacy of Autologous Bone Marrow Aspirate Transplantation Combined with Human Parathyroid Hormone 1-34 Administration to Treat Osteonecrosis in a Rabbit Model.

Feasibility and Efficacy of Autologous Bone Marrow Aspirate Transplantation Combined with Human Parathyroid Hormone 1-34 Administration to Treat Osteonecrosis in a Rabbit Model.

No studies have examined the transplantation of a bone marrow aspirate (BMA) containing mesenchymal stem cells (MSCs) combined with human parathyroid hormone 1-34 (hPTH1-34) administration. Therefore, we evaluated the feasibility and efficacy of autologous BMA transplantation combined with hPHT1-34 administration in a bone necrosis model. The metatarsal bones of rabbits were necrotized using liquid nitrogen, and the rabbits received a BMA transplantation or saline injection followed by hPTH1-34 (30 μg/kg) or saline administration three times per week (n = 3-4 per group). The rabbits were euthanized at 12 weeks after the initiation of treatment. No systemic adverse effects or local neoplastic lesions were observed. Importantly, the rabbits in the BMA transplantation plus hPTH1-34 group showed the highest bone volumes and histological scores of new bone. These data confirmed the feasibility of BMA transplantation combined with hPTH1-34, at least during the experimental period. The observed efficacy may be explained by a synergistic effect from the stimulation of MSC differentiation to osteoblasts with hPTH1-34-mediated suppression of apoptosis in osteoblasts. These results indicate the promising potential for BMA transplantation combined with hPTH1-34 administration in bone necrosis treatment. Longer term experiments are needed to confirm the safety of this therapeutic strategy.

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