视网膜A2A和A3腺苷受体调节大鼠视网膜电图成分。

IF 1.1 4区 医学 Q4 NEUROSCIENCES
Gudmundur Jonsson, Thor Eysteinsson
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引用次数: 5

摘要

腺苷是一种神经调节剂,存在于中枢神经系统的各个区域,包括视网膜。根据大鼠视网膜的视网膜电图(ERG)记录,腺苷可能在视网膜中起神经保护作用。我们的目的是评估A2A和A3腺苷受体在大鼠ERG的产生和调节中的作用。用Sprague Dawley大鼠角膜电极记录闪光灯ERG。玻璃体注射A2A和A3受体的激动剂和拮抗剂,腺苷(5µl)。考察了对单闪暗变和光变ergg组分的影响,以及ERG闪烁的影响。腺苷(0.5 mM)增加了暗位ERG a波(68±8 ~ 97±14µV, P = 0.042)和b波(236±38µV ~ 305±42µV)的平均振幅。A2A激动剂CGS21680 (2 mM)使ERG b波平均振幅从最亮刺激时的298±21µV降低到212±19µV (P = 0.005),平均暗位振荡电位(OPs)从100±9µV降低到47±11µV (P = 0.023)。A2A拮抗剂ZM241385 [4 mM]降低ERG暗位b波。A3激动剂2-CI-IB-MECA (0.5 mM)增加了a波,降低了暗位和光位ERG b波以及暗位OPs。A3拮抗剂VUF5574 (1 mM)增加暗斑a波平均振幅(66±8 ~ 140±29µV, P = 0.046)和b波平均振幅(224±20 ~ 312±39µV, P = 0.0037)。未发现对ERG闪烁有显著影响。我们得出结论,含有A2A和/或A3腺苷受体的视网膜神经元有助于ERG a波和b波以及OPs的产生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Retinal A2A and A3 adenosine receptors modulate the components of the rat electroretinogram.

Adenosine is a neuromodulator present in various areas of the central nervous system, including the retina. Adenosine may serve a neuroprotective role in the retina, based on electroretinogram (ERG) recordings from the rat retina. Our purpose was to assess the role of A2A and A3 adenosine receptors in the generation and modulation of the rat ERG. The flash ERG was recorded with corneal electrodes from Sprague Dawley rats. Agonists and antagonists for A2A and A3 receptors, and adenosine were injected (5 µl) into the vitreous. The effects on the components of the single flash scotopic and photopic ERGs were examined, and ERG flicker. Adenosine (0.5 mM) increased the mean amplitudes of the scotopic ERG a-waves (68 ± 8 to 97 ± 14 µV, P = 0.042), and b-waves (236 ± 38 µV to 305 ± 42 µV). A2A agonist CGS21680 (2 mM) reduced the mean amplitude of the ERG b-wave, from 298 ± 21 µV in response to the brightest stimulus to 212 ± 19 µV (P = 0.005), and mean scotopic oscillatory potentials (OPs) from 100 ± 9 µV to 47 ± 11 µV (P = 0.023). ZM241385 [4 mM], an A2A antagonist, decreased the scotopic b-wave of the ERG. A3 agonist 2-CI-IB-MECA (0.5 mM) increased the a-wave, while decreasing the scotopic and photopic ERG b-waves, and the scotopic OPs. A3 antagonist VUF5574 (1 mM) increased the mean amplitude of the scotopic a-wave (66 ± 8 to 140 ± 29 µV, P = 0.046) and b-wave (224 ± 20 to 312 ± 39 µV, P = 0.0037). No significant effects on ERG flicker were found. We conclude that retinal neurons containing A2A and/or A3 adenosine receptors contribute to the generation of the ERG a- and b-waves and OPs.

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来源期刊
Visual Neuroscience
Visual Neuroscience 医学-神经科学
CiteScore
2.20
自引率
5.30%
发文量
8
审稿时长
>12 weeks
期刊介绍: Visual Neuroscience is an international journal devoted to the publication of experimental and theoretical research on biological mechanisms of vision. A major goal of publication is to bring together in one journal a broad range of studies that reflect the diversity and originality of all aspects of neuroscience research relating to the visual system. Contributions may address molecular, cellular or systems-level processes in either vertebrate or invertebrate species. The journal publishes work based on a wide range of technical approaches, including molecular genetics, anatomy, physiology, psychophysics and imaging, and utilizing comparative, developmental, theoretical or computational approaches to understand the biology of vision and visuo-motor control. The journal also publishes research seeking to understand disorders of the visual system and strategies for restoring vision. Studies based exclusively on clinical, psychophysiological or behavioral data are welcomed, provided that they address questions concerning neural mechanisms of vision or provide insight into visual dysfunction.
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