柯达热球菌静止期蛋白质组和脂质组的研究。

IF 2.3 4区 生物学 Q3 MICROBIOLOGY
Archaea-An International Microbiological Journal Pub Date : 2016-05-04 eCollection Date: 2016-01-01 DOI:10.1155/2016/5938289
Emma J Gagen, Marcos Y Yoshinaga, Franka Garcia Prado, Kai-Uwe Hinrichs, Michael Thomm
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引用次数: 6

摘要

在自然界中,由于大多数环境中营养物质的限制,大多数细胞可能处于静止相状态。对细菌和酵母的研究揭示了在整个固定阶段的形态和生理变化,这导致了长期营养限制的生存能力增强。然而,关于古细菌固定相反应的信息很少。我们研究了柯达热球菌在固定期延长时间后蛋白质和脂质水平的变化。在固定阶段对时间的适应包括增加带有四醚骨架的膜脂的比例,确保翻译保真度的蛋白质合成,ABC转运蛋白的特异性调节(一些上调,另一些下调),以及参与辅酶生产的蛋白质的上调。考虑到四醚合成的生物学机制尚不清楚,我们也考虑了在同一时期内,T. kodakarensis中蛋白质水平的变化是否可能揭示四醚脂质的产生。假设的碳氮水解酶、TldE(大肠杆菌中的一种蛋白酶)同源物和膜结合氢化酶复合物亚基可能参与四醚相关反应,而腺苷钴胺素合成蛋白的上调可能支持自由基机制作为四醚合成的触发因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The Proteome and Lipidome of Thermococcus kodakarensis across the Stationary Phase.

The Proteome and Lipidome of Thermococcus kodakarensis across the Stationary Phase.

The majority of cells in nature probably exist in a stationary-phase-like state, due to nutrient limitation in most environments. Studies on bacteria and yeast reveal morphological and physiological changes throughout the stationary phase, which lead to an increased ability to survive prolonged nutrient limitation. However, there is little information on archaeal stationary phase responses. We investigated protein- and lipid-level changes in Thermococcus kodakarensis with extended time in the stationary phase. Adaptations to time in stationary phase included increased proportion of membrane lipids with a tetraether backbone, synthesis of proteins that ensure translational fidelity, specific regulation of ABC transporters (upregulation of some, downregulation of others), and upregulation of proteins involved in coenzyme production. Given that the biological mechanism of tetraether synthesis is unknown, we also considered whether any of the protein-level changes in T. kodakarensis might shed light on the production of tetraether lipids across the same period. A putative carbon-nitrogen hydrolase, a TldE (a protease in Escherichia coli) homologue, and a membrane bound hydrogenase complex subunit were candidates for possible involvement in tetraether-related reactions, while upregulation of adenosylcobalamin synthesis proteins might lend support to a possible radical mechanism as a trigger for tetraether synthesis.

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来源期刊
CiteScore
7.50
自引率
0.00%
发文量
1
审稿时长
>12 weeks
期刊介绍: Archaea is a peer-reviewed, Open Access journal that publishes original research articles as well as review articles dealing with all aspects of archaea, including environmental adaptation, enzymology, genetics and genomics, metabolism, molecular biology, molecular ecology, phylogeny, and ultrastructure. Bioinformatics studies and biotechnological implications of archaea will be considered. Published since 2002, Archaea provides a unique venue for exchanging information about these extraordinary prokaryotes.
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