蛋白质浓度测定方法

Q2 Pharmacology, Toxicology and Pharmaceutics

Current Protocols in Pharmacology Pub Date : 2016-06-01 DOI:10.1002/cpph.3

Bradley J.S.C. Olson

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引用次数: 48

摘要

蛋白质的生化分析依赖于蛋白质浓度的精确定量。本附录中详细介绍了一些常用的蛋白质分析方法，例如Lowry, Bradford, bicinchoninic acid (BCA)， UV光谱和3-(4-羧基苯甲酰)喹啉-2-甲醛(CBQCA)测定。本报告的主要重点是分析选择，强调样品和缓冲液的兼容性。考虑了生成蛋白质测定标准曲线和数据处理的基本原理，以及更常用的蛋白质测定的高通量适应性。还包括SDS-PAGE样品缓冲液中总蛋白的快速，廉价，可靠的BCA测定，用于等量装载SDS-PAGE凝胶。©2016 by John Wiley &儿子,Inc。

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Assays for Determination of Protein Concentration

Biochemical analysis of proteins relies on accurate quantification of protein concentration. Detailed in this appendix are some commonly used methods for protein analysis, e.g., Lowry, Bradford, bicinchoninic acid (BCA), UV spectroscopic, and 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA) assays. The primary focus of this report is assay selection, emphasizing sample and buffer compatibility. The fundamentals of generating protein assay standard curves and of data processing are considered, as are high-throughput adaptations of the more commonly used protein assays. Also included is a rapid, inexpensive, and reliable BCA assay of total protein in SDS-PAGE sample buffer that is used for equal loading of SDS-PAGE gels. © 2016 by John Wiley & Sons, Inc.

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来源期刊

Current Protocols in Pharmacology Pharmacology, Toxicology and Pharmaceutics-Pharmacology

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