环的化学和生物生产。

4区 生物学 Q2 Agricultural and Biological Sciences
Yilong Li, Tao Bi, Julio A Camarero
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引用次数: 27

摘要

环核苷酸是一种令人着迷的天然微蛋白(约30个残基长),存在于几个植物科中,具有多种生物学特性,如蛋白酶抑制、抗菌、杀虫、细胞毒、抗hiv和激素样活性。环核苷酸共享一个独特的头到尾的环状结拓扑结构,由三个二硫化物桥组成,其中一个二硫化物穿过由另外两个二硫化物和相互连接的肽骨架形成的大环,形成所谓的胱氨酸结拓扑结构。这种环胱氨酸结(CCK)框架赋予环聚糖特殊的刚性,耐热性和化学变性,以及抗降解酶的稳定性。有趣的是,环聚糖已被证明是口服生物可利用的,而其他环聚糖已被证明可以穿过细胞膜。此外,最近的报道还表明,工程环聚糖可以有效地用于靶向细胞外和细胞内蛋白质-蛋白质相互作用,因此使环聚糖成为药物开发的理想工具,可以选择性地靶向蛋白质-蛋白质相互作用。在这项工作中,我们将回顾所有可用的方法,生产这些有趣的蛋白质使用化学或生物方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Chemical and biological production of cyclotides.

Chemical and biological production of cyclotides.

Chemical and biological production of cyclotides.

Chemical and biological production of cyclotides.

Cyclotides are fascinating naturally occurring micro-proteins (≈30 residues long) present in several plant families, and display various biological properties such as protease inhibitory, anti-microbial, insecticidal, cytotoxic, anti-HIV and hormone-like activities. Cyclotides share a unique head-to-tail circular knotted topology of three disulfide bridges, with one disulfide penetrating through a macrocycle formed by the two other disulfides and interconnecting peptide backbones, forming what is called a cystine knot topology. This cyclic cystine knot (CCK) framework gives the cyclotides exceptional rigidity, resistance to thermal and chemical denaturation, and enzymatic stability against degradation. Interestingly, cyclotides have been shown to be orally bioavailable, and other cyclotides have been shown to cross the cell membranes. Moreover, recent reports have also shown that engineered cyclotides can be efficiently used to target extracellular and intracellular protein-protein interactions, therefore making cyclotides ideal tools for drug development to selectively target protein-protein interactions. In this work we will review all the available methods for production of these interesting proteins using chemical or biological methods.

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来源期刊
Advances in Botanical Research
Advances in Botanical Research 生物-植物科学
CiteScore
4.20
自引率
0.00%
发文量
75
审稿时长
>12 weeks
期刊介绍: Advances in Botanical Research (including Advances in Plant Pathology) have been published since 1963. The series has surpassed 85 volumes and features a wide range of reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. This includes a comprehensive series of volumes dealing with the genome evolution of plants. Recent developments on plant metabolomics have also been described.
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