植物寡糖增强小麦对紫斑病的防御反应。

L Somai-Jemmali, A Siah, B Randoux, Ph Reignault, P Halama, R Rodriguez, W Hamada
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引用次数: 0

摘要

本研究首次证实了以低聚糖(20%)为基础的新配方(Oligos)对面包小麦(BW)和硬粒小麦(DW)的主要病原菌graminicola的诱导和保护作用。在植物中,Oligos处理显著降低菌丝生长、渗透、叶肉定植和结果。在坏死阶段,Oligos还显著降低了两种小麦的CWDE活性,如木聚糖酶和葡聚糖酶以及蛋白酶活性,表明它们与疾病严重程度相关。在植物防御标记方面,PR2、Chi 4前体、Per和LOX-1编码基因上调,同时诱导葡聚糖酶(GLUC)、过氧化氢酶(CAT)和脂氧合酶(LOX)活性和总酚化合物(PC)积累。在接种环境下,H2O2和PC在真菌渗透部位局部积累,并特异性诱导苯丙氨酸解氨酶(PAL)酶活性。此外,我们的实验还显示了两种小麦的反应有一些相似之处和差异。DW叶片对GLUC、CAT活性和H2O2积累的响应更强,而BW叶片对LOX、PAL活性和PC积累的响应更早、更强。所测试的Oligos配方在小麦品种中均表现出高且稳定的抗性诱导活性,表明其在小麦重和体重上均可用于常见的STB防治策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
PLANT OLIGOSACCHARIDES ENHANCE WHEAT DEFENCE RESPONSE AGAINST SEPTORIA LEAF BLOTCH.

Our work provides the first evidence for elicitation and protection effects of preventive treatments with oligosaccharides (20%)-based new formulation (Oligos) against Mycosphaerella graminicola, a major pathogen of bread wheat (BW) and durum wheat (DW). In planta Oligos treatment led to strongly reduced hyphal growth, penetration, mesophyll colonization and fructification. During the necrotrophic phase, Oligos also drastically decreased the production of M. graminicola CWDE activities, such as xylanase and glucanase as well as protease activity in both wheat species, suggesting their correlation with disease severity. Concerning plant defence markers, PR2, Chi 4 precursor-, Per- and LOX-1-encoding genes were up-regulated, while glucanase (GLUC), catalase (CAT) and lipoxygenase (LOX) activities and total phenolic compound (PC) accumulation were induced in both (non-inoculated and inoculated contexts. In inoculated context, a localized accumulation of H2O2 and PC at fungal penetration sites and a specific induction of phenylalanine ammonia-Lyase (PAL) enzymatic activity were observed. Moreover, our experiment exhibited some similarities and differences in both wheat species responses. GLUC and CAT activities and H2O2 accumulation were more responsive in DW leaves, while LOX and PAL activities and PC accumulation occurred earlier and to a stronger extent in BW leaves. The tested Oligos formulation showed an interesting resistance induction activity characterized by a high and stable efficiency whatever the wheat species, suggesting it integration in common control strategies against STB on both DW and BW.

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