Lenka Sedlaříková, Lenka Bešše, Soňa Novosadová, Veronika Kubaczková, Lenka Radová, Michal Staník, Marta Krejčí, Roman Hájek, Sabina Ševčíková
{"title":"尿液中的microrna不是多发性骨髓瘤的生物标志物。","authors":"Lenka Sedlaříková, Lenka Bešše, Soňa Novosadová, Veronika Kubaczková, Lenka Radová, Michal Staník, Marta Krejčí, Roman Hájek, Sabina Ševčíková","doi":"10.1186/s12952-015-0035-7","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>In this study, we aimed to identify microRNA from urine of multiple myeloma patients that could serve as a biomarker for the disease.</p><p><strong>Results: </strong>Analysis of urine samples was performed using Serum/Plasma Focus PCR MicroRNA Panel (Exiqon) and verified using individual TaqMan miRNA assays for qPCR. We found 20 deregulated microRNA (p < 0.05); for further validation, we chose 8 of them. Nevertheless, only differences in expression levels of miR-22-3p remained close to statistical significance.</p><p><strong>Conclusions: </strong>Our preliminary results did not confirm urine microRNA as a potential biomarker for multiple myeloma.</p>","PeriodicalId":73849,"journal":{"name":"Journal of negative results in biomedicine","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2015-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12952-015-0035-7","citationCount":"7","resultStr":"{\"title\":\"MicroRNAs in urine are not biomarkers of multiple myeloma.\",\"authors\":\"Lenka Sedlaříková, Lenka Bešše, Soňa Novosadová, Veronika Kubaczková, Lenka Radová, Michal Staník, Marta Krejčí, Roman Hájek, Sabina Ševčíková\",\"doi\":\"10.1186/s12952-015-0035-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>In this study, we aimed to identify microRNA from urine of multiple myeloma patients that could serve as a biomarker for the disease.</p><p><strong>Results: </strong>Analysis of urine samples was performed using Serum/Plasma Focus PCR MicroRNA Panel (Exiqon) and verified using individual TaqMan miRNA assays for qPCR. We found 20 deregulated microRNA (p < 0.05); for further validation, we chose 8 of them. Nevertheless, only differences in expression levels of miR-22-3p remained close to statistical significance.</p><p><strong>Conclusions: </strong>Our preliminary results did not confirm urine microRNA as a potential biomarker for multiple myeloma.</p>\",\"PeriodicalId\":73849,\"journal\":{\"name\":\"Journal of negative results in biomedicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1186/s12952-015-0035-7\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of negative results in biomedicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s12952-015-0035-7\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of negative results in biomedicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s12952-015-0035-7","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
MicroRNAs in urine are not biomarkers of multiple myeloma.
Background: In this study, we aimed to identify microRNA from urine of multiple myeloma patients that could serve as a biomarker for the disease.
Results: Analysis of urine samples was performed using Serum/Plasma Focus PCR MicroRNA Panel (Exiqon) and verified using individual TaqMan miRNA assays for qPCR. We found 20 deregulated microRNA (p < 0.05); for further validation, we chose 8 of them. Nevertheless, only differences in expression levels of miR-22-3p remained close to statistical significance.
Conclusions: Our preliminary results did not confirm urine microRNA as a potential biomarker for multiple myeloma.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
