Lenka Sedlaříková, Lenka Bešše, Soňa Novosadová, Veronika Kubaczková, Lenka Radová, Michal Staník, Marta Krejčí, Roman Hájek, Sabina Ševčíková
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MicroRNAs in urine are not biomarkers of multiple myeloma.
Background: In this study, we aimed to identify microRNA from urine of multiple myeloma patients that could serve as a biomarker for the disease.
Results: Analysis of urine samples was performed using Serum/Plasma Focus PCR MicroRNA Panel (Exiqon) and verified using individual TaqMan miRNA assays for qPCR. We found 20 deregulated microRNA (p < 0.05); for further validation, we chose 8 of them. Nevertheless, only differences in expression levels of miR-22-3p remained close to statistical significance.
Conclusions: Our preliminary results did not confirm urine microRNA as a potential biomarker for multiple myeloma.
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