糖尿病视网膜病变:糖尿病啮齿动物的视网膜特异性维持方法和血管组织病理学和分子异常的评估。

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2015-09-01 DOI:10.1002/9780470942390.mo140190

Alexander Veenstra, Haitao Liu, Chieh Allen Lee, Yunpeng Du, Jie Tang, Timothy S Kern

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引用次数: 44

摘要

糖尿病视网膜病变是视力损害的主要原因，随着越来越多的人患上糖尿病，其患病率继续增加。尽管视力很重要，但视网膜是人体最小的组织之一，并且已经开发出专门的技术来研究视网膜病变。本文总结了几种方法，用于(i)诱导小鼠糖尿病，(ii)在典型血管组织病理学发展所需的几个月内维持糖尿病动物，(iii)评估糖尿病视网膜病变的血管组织病理学，以及(iv)量化与视网膜病变发展有关的异常。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Diabetic Retinopathy: Retina-Specific Methods for Maintenance of Diabetic Rodents and Evaluation of Vascular Histopathology and Molecular Abnormalities.

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Diabetic Retinopathy: Retina-Specific Methods for Maintenance of Diabetic Rodents and Evaluation of Vascular Histopathology and Molecular Abnormalities.

Diabetic retinopathy is a major cause of visual impairment, which continues to increase in prevalence as more and more people develop diabetes. Despite the importance of vision, the retina is one of the smallest tissues in the body, and specialized techniques have been developed to study retinopathy. This article summarizes several methods used to (i) induce diabetes in mice, (ii) maintain the diabetic animals throughout the months required for development of typical vascular histopathology, (iii) evaluate vascular histopathology of diabetic retinopathy, and (iv) quantitate abnormalities implicated in the development of the retinopathy.

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来源期刊

Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.