黄曲霉的转录组分析揭示了vea依赖性次级代谢产物基因簇的调控,包括新的黄素簇。

Eukaryotic Cell Pub Date : 2015-10-01 Epub Date: 2015-07-24 DOI:10.1128/EC.00092-15
J W Cary, Z Han, Y Yin, J M Lohmar, S Shantappa, P Y Harris-Coward, B Mack, K C Ehrlich, Q Wei, N Arroyo-Manzanares, V Uka, L Vanhaecke, D Bhatnagar, J Yu, W C Nierman, M A Johns, D Sorensen, H Shen, S De Saeger, J Diana Di Mavungu, A M Calvo
{"title":"黄曲霉的转录组分析揭示了vea依赖性次级代谢产物基因簇的调控,包括新的黄素簇。","authors":"J W Cary,&nbsp;Z Han,&nbsp;Y Yin,&nbsp;J M Lohmar,&nbsp;S Shantappa,&nbsp;P Y Harris-Coward,&nbsp;B Mack,&nbsp;K C Ehrlich,&nbsp;Q Wei,&nbsp;N Arroyo-Manzanares,&nbsp;V Uka,&nbsp;L Vanhaecke,&nbsp;D Bhatnagar,&nbsp;J Yu,&nbsp;W C Nierman,&nbsp;M A Johns,&nbsp;D Sorensen,&nbsp;H Shen,&nbsp;S De Saeger,&nbsp;J Diana Di Mavungu,&nbsp;A M Calvo","doi":"10.1128/EC.00092-15","DOIUrl":null,"url":null,"abstract":"<p><p>The global regulatory veA gene governs development and secondary metabolism in numerous fungal species, including Aspergillus flavus. This is especially relevant since A. flavus infects crops of agricultural importance worldwide, contaminating them with potent mycotoxins. The most well-known are aflatoxins, which are cytotoxic and carcinogenic polyketide compounds. The production of aflatoxins and the expression of genes implicated in the production of these mycotoxins are veA dependent. The genes responsible for the synthesis of aflatoxins are clustered, a signature common for genes involved in fungal secondary metabolism. Studies of the A. flavus genome revealed many gene clusters possibly connected to the synthesis of secondary metabolites. Many of these metabolites are still unknown, or the association between a known metabolite and a particular gene cluster has not yet been established. In the present transcriptome study, we show that veA is necessary for the expression of a large number of genes. Twenty-eight out of the predicted 56 secondary metabolite gene clusters include at least one gene that is differentially expressed depending on presence or absence of veA. One of the clusters under the influence of veA is cluster 39. The absence of veA results in a downregulation of the five genes found within this cluster. Interestingly, our results indicate that the cluster is expressed mainly in sclerotia. Chemical analysis of sclerotial extracts revealed that cluster 39 is responsible for the production of aflavarin. </p>","PeriodicalId":11891,"journal":{"name":"Eukaryotic Cell","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2015-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/EC.00092-15","citationCount":"62","resultStr":"{\"title\":\"Transcriptome Analysis of Aspergillus flavus Reveals veA-Dependent Regulation of Secondary Metabolite Gene Clusters, Including the Novel Aflavarin Cluster.\",\"authors\":\"J W Cary,&nbsp;Z Han,&nbsp;Y Yin,&nbsp;J M Lohmar,&nbsp;S Shantappa,&nbsp;P Y Harris-Coward,&nbsp;B Mack,&nbsp;K C Ehrlich,&nbsp;Q Wei,&nbsp;N Arroyo-Manzanares,&nbsp;V Uka,&nbsp;L Vanhaecke,&nbsp;D Bhatnagar,&nbsp;J Yu,&nbsp;W C Nierman,&nbsp;M A Johns,&nbsp;D Sorensen,&nbsp;H Shen,&nbsp;S De Saeger,&nbsp;J Diana Di Mavungu,&nbsp;A M Calvo\",\"doi\":\"10.1128/EC.00092-15\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The global regulatory veA gene governs development and secondary metabolism in numerous fungal species, including Aspergillus flavus. This is especially relevant since A. flavus infects crops of agricultural importance worldwide, contaminating them with potent mycotoxins. The most well-known are aflatoxins, which are cytotoxic and carcinogenic polyketide compounds. The production of aflatoxins and the expression of genes implicated in the production of these mycotoxins are veA dependent. The genes responsible for the synthesis of aflatoxins are clustered, a signature common for genes involved in fungal secondary metabolism. Studies of the A. flavus genome revealed many gene clusters possibly connected to the synthesis of secondary metabolites. Many of these metabolites are still unknown, or the association between a known metabolite and a particular gene cluster has not yet been established. In the present transcriptome study, we show that veA is necessary for the expression of a large number of genes. Twenty-eight out of the predicted 56 secondary metabolite gene clusters include at least one gene that is differentially expressed depending on presence or absence of veA. One of the clusters under the influence of veA is cluster 39. The absence of veA results in a downregulation of the five genes found within this cluster. Interestingly, our results indicate that the cluster is expressed mainly in sclerotia. Chemical analysis of sclerotial extracts revealed that cluster 39 is responsible for the production of aflavarin. </p>\",\"PeriodicalId\":11891,\"journal\":{\"name\":\"Eukaryotic Cell\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1128/EC.00092-15\",\"citationCount\":\"62\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Eukaryotic Cell\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1128/EC.00092-15\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2015/7/24 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Eukaryotic Cell","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1128/EC.00092-15","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2015/7/24 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 62

摘要

全球调控veA基因控制着许多真菌物种的发育和次生代谢,包括黄曲霉。这是特别相关的,因为黄曲霉感染世界各地的重要农业作物,用强效真菌毒素污染它们。最著名的是黄曲霉毒素,这是一种细胞毒性和致癌的聚酮化合物。黄曲霉毒素的产生和与这些真菌毒素产生有关的基因的表达是依赖于veA的。负责黄曲霉毒素合成的基因聚集在一起,这是参与真菌次级代谢的基因的共同特征。黄曲霉基因组的研究揭示了许多可能与次生代谢物合成有关的基因簇。其中许多代谢物仍然是未知的,或者已知代谢物与特定基因簇之间的关联尚未确定。在目前的转录组研究中,我们发现veA是大量基因表达所必需的。在预测的56个次级代谢物基因簇中,有28个包括至少一个因存在或不存在veA而差异表达的基因。受veA影响的集群之一是集群39。缺乏veA会导致该基因簇中5个基因的下调。有趣的是,我们的结果表明该簇主要在菌核中表达。对菌核提取物的化学分析表明,簇39负责黄素的产生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Transcriptome Analysis of Aspergillus flavus Reveals veA-Dependent Regulation of Secondary Metabolite Gene Clusters, Including the Novel Aflavarin Cluster.

Transcriptome Analysis of Aspergillus flavus Reveals veA-Dependent Regulation of Secondary Metabolite Gene Clusters, Including the Novel Aflavarin Cluster.

Transcriptome Analysis of Aspergillus flavus Reveals veA-Dependent Regulation of Secondary Metabolite Gene Clusters, Including the Novel Aflavarin Cluster.

The global regulatory veA gene governs development and secondary metabolism in numerous fungal species, including Aspergillus flavus. This is especially relevant since A. flavus infects crops of agricultural importance worldwide, contaminating them with potent mycotoxins. The most well-known are aflatoxins, which are cytotoxic and carcinogenic polyketide compounds. The production of aflatoxins and the expression of genes implicated in the production of these mycotoxins are veA dependent. The genes responsible for the synthesis of aflatoxins are clustered, a signature common for genes involved in fungal secondary metabolism. Studies of the A. flavus genome revealed many gene clusters possibly connected to the synthesis of secondary metabolites. Many of these metabolites are still unknown, or the association between a known metabolite and a particular gene cluster has not yet been established. In the present transcriptome study, we show that veA is necessary for the expression of a large number of genes. Twenty-eight out of the predicted 56 secondary metabolite gene clusters include at least one gene that is differentially expressed depending on presence or absence of veA. One of the clusters under the influence of veA is cluster 39. The absence of veA results in a downregulation of the five genes found within this cluster. Interestingly, our results indicate that the cluster is expressed mainly in sclerotia. Chemical analysis of sclerotial extracts revealed that cluster 39 is responsible for the production of aflavarin.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Eukaryotic Cell
Eukaryotic Cell 生物-微生物学
自引率
0.00%
发文量
0
审稿时长
1 months
期刊介绍: Eukaryotic Cell (EC) focuses on eukaryotic microbiology and presents reports of basic research on simple eukaryotic microorganisms, such as yeasts, fungi, algae, protozoa, and social amoebae. The journal also covers viruses of these organisms and their organelles and their interactions with other living systems, where the focus is on the eukaryotic cell. Topics include: - Basic biology - Molecular and cellular biology - Mechanisms, and control, of developmental pathways - Structure and form inherent in basic biological processes - Cellular architecture - Metabolic physiology - Comparative genomics, biochemistry, and evolution - Population dynamics - Ecology
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信