真菌、微藻和地衣的酶体超微结构和进化。

Eukaryotic Cell Pub Date : 2015-10-01 Epub Date: 2015-08-07 DOI:10.1128/EC.00106-15
Jae-Hyeok Lee, John E Heuser, Robyn Roth, Ursula Goodenough
{"title":"真菌、微藻和地衣的酶体超微结构和进化。","authors":"Jae-Hyeok Lee,&nbsp;John E Heuser,&nbsp;Robyn Roth,&nbsp;Ursula Goodenough","doi":"10.1128/EC.00106-15","DOIUrl":null,"url":null,"abstract":"<p><p>Eisosomes are among the few remaining eukaryotic cellular differentations that lack a defined function(s). These trough-shaped invaginations of the plasma membrane have largely been studied in Saccharomyces cerevisiae, in which their associated proteins, including two BAR domain proteins, have been identified, and homologues have been found throughout the fungal radiation. Using quick-freeze deep-etch electron microscopy to generate high-resolution replicas of membrane fracture faces without the use of chemical fixation, we report that eisosomes are also present in a subset of red and green microalgae as well as in the cysts of the ciliate Euplotes. Eisosome assembly is closely correlated with both the presence and the nature of cell walls. Microalgal eisosomes vary extensively in topology and internal organization. Unlike fungi, their convex fracture faces can carry lineage-specific arrays of intramembranous particles, and their concave fracture faces usually display fine striations, also seen in fungi, that are pitched at lineage-specific angles and, in some cases, adopt a broad-banded patterning. The conserved genes that encode fungal eisosome-associated proteins are not found in sequenced algal genomes, but we identified genes encoding two algal lineage-specific families of predicted BAR domain proteins, called Green-BAR and Red-BAR, that are candidate eisosome organizers. We propose a model for eisosome formation wherein (i) positively charged recognition patches first establish contact with target membrane regions and (ii) a (partial) unwinding of the coiled-coil conformation of the BAR domains then allows interactions between the hydrophobic faces of their amphipathic helices and the lipid phase of the inner membrane leaflet, generating the striated patterns. </p>","PeriodicalId":11891,"journal":{"name":"Eukaryotic Cell","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2015-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/EC.00106-15","citationCount":"37","resultStr":"{\"title\":\"Eisosome Ultrastructure and Evolution in Fungi, Microalgae, and Lichens.\",\"authors\":\"Jae-Hyeok Lee,&nbsp;John E Heuser,&nbsp;Robyn Roth,&nbsp;Ursula Goodenough\",\"doi\":\"10.1128/EC.00106-15\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Eisosomes are among the few remaining eukaryotic cellular differentations that lack a defined function(s). These trough-shaped invaginations of the plasma membrane have largely been studied in Saccharomyces cerevisiae, in which their associated proteins, including two BAR domain proteins, have been identified, and homologues have been found throughout the fungal radiation. Using quick-freeze deep-etch electron microscopy to generate high-resolution replicas of membrane fracture faces without the use of chemical fixation, we report that eisosomes are also present in a subset of red and green microalgae as well as in the cysts of the ciliate Euplotes. Eisosome assembly is closely correlated with both the presence and the nature of cell walls. Microalgal eisosomes vary extensively in topology and internal organization. Unlike fungi, their convex fracture faces can carry lineage-specific arrays of intramembranous particles, and their concave fracture faces usually display fine striations, also seen in fungi, that are pitched at lineage-specific angles and, in some cases, adopt a broad-banded patterning. The conserved genes that encode fungal eisosome-associated proteins are not found in sequenced algal genomes, but we identified genes encoding two algal lineage-specific families of predicted BAR domain proteins, called Green-BAR and Red-BAR, that are candidate eisosome organizers. We propose a model for eisosome formation wherein (i) positively charged recognition patches first establish contact with target membrane regions and (ii) a (partial) unwinding of the coiled-coil conformation of the BAR domains then allows interactions between the hydrophobic faces of their amphipathic helices and the lipid phase of the inner membrane leaflet, generating the striated patterns. </p>\",\"PeriodicalId\":11891,\"journal\":{\"name\":\"Eukaryotic Cell\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1128/EC.00106-15\",\"citationCount\":\"37\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Eukaryotic Cell\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1128/EC.00106-15\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2015/8/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Eukaryotic Cell","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1128/EC.00106-15","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2015/8/7 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 37

摘要

溶酶体是现存的真核生物中为数不多的缺乏明确功能的细胞差异。这些槽状的质膜内陷已经在酿酒酵母中进行了大量的研究,其中它们的相关蛋白,包括两个BAR结构域蛋白,已经被鉴定出来,并且在真菌辐射中发现了同源物。使用速冻深蚀刻电子显微镜在不使用化学固定的情况下生成膜断裂面的高分辨率复制品,我们报告说,在红色和绿色微藻的一个亚群中以及在纤毛虫的包囊中也存在同种体。酶体的组装与细胞壁的存在和性质密切相关。微藻酶体在拓扑结构和内部组织上有很大的不同。与真菌不同的是,它们的凸断裂面可以携带谱系特异性的膜内颗粒阵列,而它们的凹断裂面通常显示出精细的条纹,这在真菌中也可以看到,这些条纹以谱系特异性的角度倾斜,在某些情况下,采用宽带状图案。在测序的藻类基因组中没有发现编码真菌eisosome相关蛋白的保守基因,但我们发现了编码两个藻类谱系特异性的预测BAR结构域蛋白家族的基因,称为Green-BAR和Red-BAR,它们是候选的eisosome组织体。我们提出了一种酶同体形成的模型,其中(i)带正电荷的识别斑块首先与靶膜区域建立接触;(ii) BAR结构域的螺旋状构象(部分)解绕,然后允许其两亲螺旋的疏水面与内膜小叶的脂质相相互作用,产生条纹图案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Eisosome Ultrastructure and Evolution in Fungi, Microalgae, and Lichens.

Eisosome Ultrastructure and Evolution in Fungi, Microalgae, and Lichens.

Eisosome Ultrastructure and Evolution in Fungi, Microalgae, and Lichens.

Eisosomes are among the few remaining eukaryotic cellular differentations that lack a defined function(s). These trough-shaped invaginations of the plasma membrane have largely been studied in Saccharomyces cerevisiae, in which their associated proteins, including two BAR domain proteins, have been identified, and homologues have been found throughout the fungal radiation. Using quick-freeze deep-etch electron microscopy to generate high-resolution replicas of membrane fracture faces without the use of chemical fixation, we report that eisosomes are also present in a subset of red and green microalgae as well as in the cysts of the ciliate Euplotes. Eisosome assembly is closely correlated with both the presence and the nature of cell walls. Microalgal eisosomes vary extensively in topology and internal organization. Unlike fungi, their convex fracture faces can carry lineage-specific arrays of intramembranous particles, and their concave fracture faces usually display fine striations, also seen in fungi, that are pitched at lineage-specific angles and, in some cases, adopt a broad-banded patterning. The conserved genes that encode fungal eisosome-associated proteins are not found in sequenced algal genomes, but we identified genes encoding two algal lineage-specific families of predicted BAR domain proteins, called Green-BAR and Red-BAR, that are candidate eisosome organizers. We propose a model for eisosome formation wherein (i) positively charged recognition patches first establish contact with target membrane regions and (ii) a (partial) unwinding of the coiled-coil conformation of the BAR domains then allows interactions between the hydrophobic faces of their amphipathic helices and the lipid phase of the inner membrane leaflet, generating the striated patterns.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Eukaryotic Cell
Eukaryotic Cell 生物-微生物学
自引率
0.00%
发文量
0
审稿时长
1 months
期刊介绍: Eukaryotic Cell (EC) focuses on eukaryotic microbiology and presents reports of basic research on simple eukaryotic microorganisms, such as yeasts, fungi, algae, protozoa, and social amoebae. The journal also covers viruses of these organisms and their organelles and their interactions with other living systems, where the focus is on the eukaryotic cell. Topics include: - Basic biology - Molecular and cellular biology - Mechanisms, and control, of developmental pathways - Structure and form inherent in basic biological processes - Cellular architecture - Metabolic physiology - Comparative genomics, biochemistry, and evolution - Population dynamics - Ecology
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信