通过生物信息学分析鉴定儿童ACC的潜在核心基因和mirna。

IF 1.1 Q2 MEDICINE, GENERAL & INTERNAL
Chunyan Fang, Yulong Ye, Fangyue Wang, Yifeng Shen, Yaodong You
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引用次数: 2

摘要

小儿肾上腺皮质癌(ACC)是一种罕见的侵袭性肿瘤,预后不均匀,通常是最致命的恶性肿瘤,其病因尚不清楚。本研究的目的是确定导致儿童ACC发展的因素,更好地了解该疾病,并研究新的分子生物标志物和治疗靶点。为了确定与儿童ACC相关的关键基因和mirna及其潜在的分子机制,我们分析了GSEGSE75415和GSE169253微阵列数据集。通过分析GSEGSE75415和GSE169253数据集,共获得329个差异产生基因(deg)和187个差异产生mirna (dem)。接下来,通过重叠dem的靶mrna获得3359个基因。通过蛋白-蛋白互作网络和基因本体分析,筛选出10个度最高的节点作为枢纽基因。其中,高表达的枢纽基因MAPK1和EP300与较差的总生存率相关。此外,hsa-miR-376、hsa-miR-148、hsa-miR-139和hsa-miR-1305与较差的生存率密切相关。我们提出中枢基因(MAPK1、EP300、hsa-miR-376、hsa-miR-148、hsa-miR-139和hsa-miR-1305)可能对肾上腺皮质肿瘤细胞的增殖和迁移有明确的影响。这些中枢基因在肾上腺皮质肿瘤中的作用可能为改善儿童ACC患者的诊断和治疗提供新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Identification of potential core genes and miRNAs in pediatric ACC <i>via</i> bioinformatics analysis.

Identification of potential core genes and miRNAs in pediatric ACC <i>via</i> bioinformatics analysis.

Identification of potential core genes and miRNAs in pediatric ACC via bioinformatics analysis.

Pediatric adrenocortical carcinomas (ACC) are rare aggressive neoplasms with heterogeneous prognosis, and often produce a most lethal malignant tumor, whereas its aetiology is still unclear. The aim of the present study was to identify the factors responsible for the development of pediatric ACC, a better understanding of the disease, and investigate new molecular biomarkers and therapeutic targets. To identify the key genes and miRNAs linked to pediatric ACC, as well as their potential molecular mechanisms, the GSEGSE75415 and GSE169253 microarray datasets were analyzed. A total of 329 differentially produced genes (DEGs) and 187 differentially produced miRNAs (DEMs) were obtained after analyzing the GSEGSE75415 and GSE169253 datasets, respectively. Next, 3,359 genes were obtained by overlapping the target mRNAs of DEMs. Following protein-protein interaction network and Gene Ontology analysis, the ten nodes with the highest degrees were screened as hub genes. Among them, the highly expressed hub genes, MAPK1 and EP300, were associated with a worse overall survival. Additionally, hsa-miR-376, hsa-miR-148, hsa-miR-139, and hsa-miR-1305 were strongly associated with poorer survival. We proposed that the hub genes (MAPK1, EP300, hsa-miR-376, hsa-miR-148, hsa-miR-139, and hsa-miR-1305) may have a definite impact on cellular proliferation and migration in adrenocortical tumors. The roles of these hub genes in adrenocortical tumors may provide novel insight to improve the diagnosis and treatment of patients with pediatric ACC.

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来源期刊
Intractable & rare diseases research
Intractable & rare diseases research MEDICINE, GENERAL & INTERNAL-
CiteScore
2.10
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29
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