金属混合物(CdCl2、HgCl2、Pb(NO3))对雄性Wistar大鼠的多器官诱导毒性及车前草茎汁的改良潜力

International journal of physiology, pathophysiology and pharmacology Pub Date : 2022-08-15 eCollection Date: 2022-01-01
Anthonet Ndidiamaka Ezejiofor, Chinna Nneka Orish, Onyewuchi Akaranta
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引用次数: 0

摘要

工业化和城市化导致各种形式的排放激增,其中许多对植物、动物、环境,甚至对人类都有有害的影响。在对新产品(家用和医疗)的追求中,世界各地的制造业不知疲倦地使用金属来满足人类的需求。然而,这些金属,特别是本研究仅限于这些金属(汞、镉和铅),不仅对环境有害,而且对人类的生命和存在也有害。30只雄性Wistar大鼠被分成6组,每组5只。制备重金属原液,根据个体体重计算所需剂量,按此给药2-5组,3 -5组在接受HMM(重金属混合物)后增加剂量给予车前草茎汁(PSJ), 6组只给予研究中使用的PSJ中剂量。大鼠体重监测每3周1次,饲料和液体摄取量监测每3周3次。在第九周结束时,将动物称重并处死。采集感兴趣的器官(脑、心、肺和胸腺)并进行分析。所做的分析包括组织病理学、血液学、生化和器官/血液金属浓度。结果显示,与正常对照组和PSJ治疗组相比,只接受金属混合物治疗的动物体重有所下降。这可能与金属诱导组采食量下降有关。然而,在胸腺和小脑的组织学上没有观察到明显的影响,即使在大脑皮层中存在液泡表明异常。心脏和肺的组织学表现出一定程度的扭曲,这种扭曲随PSJ的剂量依赖性而得到改善。有趣的是,在服用金属混合物后抗氧化水平下降后,随着PSJ剂量的增加,观察到增强效应。总之,最近的研究结果表明,用PSJ治疗HMM中毒,通过调节血液生化参数和氧化应激水平,在保护动物免受所有可能的器官毒性方面发挥了重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multi-Organ inducedtoxicity of metal mixture (CdCl2, HgCl2, Pb(NO3)), and the ameliorative potentials of plantain Musa paradisiaca (F. Musaceae) stem juice on male Wistar rats.

Industrialization and urbanization have caused a hike in all forms of emissions, many of which have detrimental effects on plants, animals, the environment, and worse still, humans. In a quest for novel products (household, and medical), manufacturing industries work tirelessly worldwide using metals to meet man's needs. However, such metals especially those confined to this research (Hg, Cd, and Pb) are inherently hazardous to not just the environment but human life and existence. Thirty (30) male Wistar rats divided into six groups of five rats each was used for the study. Stock solutions of the heavy metals were prepared and the required dose calculated according to individual weight and administered as such to group 2-5, plantain stem juice (PSJ) was administered to groups 3 to 5 in increasing dose after receiving the HMM (heavy metal mixture) while group six received medium-dose of PSJ used in the study only. Bodyweight of the rats was monitored once in three weeks while the feed and fluid intake were monitored thrice a week. At the end of the ninth week, the animals were weighed and sacrificed. Organs of interest (brain, heart, lungs, and thymus) were harvested and analyzed. Analysis done include Histopathology, hematological, biochemical, and organs/blood metal concentration. The results obtained showed a decline in the weight of animals that received metal mixture only when compared to normal control and PSJ treated groups. This could be traceable to the decline in feed intake of the metal-induced groups. However, no significant effect was observed in the histology of the Thymus and cerebellum even though the presence of a vacuole in the cerebral cortex indicated an anomaly. The histology of the heart and the lungs showed some level of distortion which was ameliorated dose-dependently with the administration of PSJ. Interestingly, after a decrease in the antioxidant level upon administration of metal mixture, a booster effect was observed with an increasing dose of PSJ. In conclusion, the recent findings have demonstrated that treatment with PSJ in HMM induced intoxication has a significant role in protecting the animals from all possible organ toxicity by modulating hemato-biochemical parameters and oxidative stress level.

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