利用荧光条形码的高通量、平行流式细胞术筛选数百种细胞表面抗原。

Q4 Biochemistry, Genetics and Molecular Biology
Stanislav Drápela, Radek Fedr, Ondřej Vacek, Ján Remšík, Karel Souček
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引用次数: 0

摘要

多色流式细胞术允许在几分钟内以单细胞分辨率分析数百万细胞中的数十个细胞参数。由于缺乏可行且相对便宜的基于抗体的方法分析如此多细胞的技术,导致我们开发了一种基于高通量细胞测定的表面分析平台。我们将荧光细胞条形码与预先存在的商业筛选工具相结合,以大规模分析细胞表面指纹。这种强大的方法将有助于识别新的生物标志物和药物靶点,并促进免疫学、肿瘤学和发育生物学中新概念的发现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding.

Multicolor flow cytometry allows for analysis of tens of cellular parameters in millions of cells at a single-cell resolution within minutes. The lack of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commercially available screening tools to analyze cell surface fingerprint at a large scale. This powerful approach will help to identify novel biomarkers and druggable targets and facilitate the discovery of new concepts in immunology, oncology, and developmental biology.

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来源期刊
Methods in molecular biology
Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
2.00
自引率
0.00%
发文量
3536
期刊介绍: For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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