经皮神经电刺激对第三腰椎横突综合征大鼠的影响。

Huan Li, Xiao-Jun Shang, Qi-Rong Dong
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引用次数: 9

摘要

目的:探讨经皮局部或远端穴位电刺激(TENS)对大鼠第三腰椎横突综合征模型的镇痛和抗炎作用。方法:将40只Sprague-Dawley大鼠随机分为对照组、模型组、模型加BL23局部穴位刺激组(模型+LAS)和模型加ST36远处穴位刺激组(模型+DAS)。除对照组外,所有大鼠在第2天进行手术第三腰椎横突综合征建模。此后,模型+LAS组和模型+DAS组大鼠从第16天至第29天每天给予TENS治疗,共6次(2/100 Hz, 30 min/天)。治疗期间每周测量一次热痛阈值,并持续到第57天,采集局部肌肉组织进行RT-PCR和组织病理学检查,并进行苏木精和伊红染色。检测白细胞介素-1β (IL-1β)、肿瘤坏死因子-α (TNF-α)和诱导型一氧化氮合酶(iNOS) mRNA表达。结果:各模型大鼠热痛阈值均较对照组降低。在治疗期间,LAS和DAS在除一个点外的所有点均显著增加热痛阈值。组织病理学检查显示,模型+LAS组和模型+DAS组大鼠第三腰椎横突周围局部肌肉组织均有不同程度恢复;然而,LAS似乎有更大的效果。模型+LAS组和模型+DAS组大鼠局部肌肉组织中IL-1β、TNF-α和iNOS mRNA表达量均升高,但均降低。LAS后的有益效果大于DAS。结论:TENS在局部(BL23)和远端(ST36)穴位对第三腰椎横突综合征大鼠均有镇痛作用,LAS的抗炎镇痛作用明显优于DAS。试验注册号:09073。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of transcutaneous electrical nerve stimulation on rats with the third lumbar vertebrae transverse process syndrome.

Objective: To investigate the analgesic and anti-inflammatory effects of transcutaneous electrical nerve stimulation (TENS) at local or distant acupuncture points in a rat model of the third lumbar vertebrae transverse process syndrome.

Methods: Forty Sprague-Dawley rats were randomly divided into control, model, model plus local acupuncture point stimulation at BL23 (model+LAS) and model plus distant acupuncture point stimulation at ST36 (model+DAS) groups. All rats except controls underwent surgical third lumbar vertebrae transverse process syndrome modelling on day 2. Thereafter, rats in the model+LAS and model+DAS groups were treated daily with TENS for a total of six treatments (2/100 Hz, 30 min/day) from day 16 to day 29. Thermal pain thresholds were measured once a week during treatment and were continued until day 57, when local muscle tissue was sampled for RT-PCR and histopathological examination after haematoxylin and eosin staining. mRNA expression of interleukin-1 β (IL-1β), tumour necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) was determined.

Results: Thermal pain thresholds of all model rats decreased relative to the control group. Both LAS and DAS significantly increased the thermal pain threshold at all but one point during the treatment period. Histopathological assessment revealed that the local muscle tissues around the third lumbar vertebrae transverse process recovered to some degree in both the model+LAS and model+DAS groups; however, LAS appeared to have a greater effect. mRNA expression of IL-1β, TNF-α and iNOS in the local muscle tissues was increased after modelling and attenuated in both model+LAS and model+DAS groups. The beneficial effect was greater after LAS than after DAS.

Conclusions: TENS at both local (BL23) and distant (ST36) acupuncture points had a pain-relieving effect in rats with the third lumbar vertebrae transverse process syndrome, and LAS appeared to have greater anti-inflammatory and analgesic effects than DAS.

Trial registration number: 09073.

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