人肺远端细胞能促进体外培养的人肺微血管内皮细胞的增殖和血管生成。

Molecular and cellular therapies Pub Date : 2014-02-01 eCollection Date: 2014-01-01
Yonghua Zheng, Xiaoke Chen, Mengjia Qian, Miaomiao Zhang, Ding Zhang, Chunxue Bai, Qun Wang, Xiangdong Wang
{"title":"人肺远端细胞能促进体外培养的人肺微血管内皮细胞的增殖和血管生成。","authors":"Yonghua Zheng, Xiaoke Chen, Mengjia Qian, Miaomiao Zhang, Ding Zhang, Chunxue Bai, Qun Wang, Xiangdong Wang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>In the previous studies, telocytes were found near the capillaries in many tissues, especially on the extracellular matrix of blood vessels and positive to CD34 and c-kit. Therefore, the present study aimed to explore if telocytes could produce angiogenesis associated cytokines, promote the proliferation and the angiogenesis of vascular endothelial cells in vitro.</p><p><strong>Methods: </strong>Human lung telocytes were isolated and cultured, and were identified by immunofluorescence cytochemistry with CD34, c-kit and vimentin. Telocytes conditional media (TCM) was prepared, and the expressions of angiogenesis associated cytokines in TCM were detected by ELISA. Human pulmonary microvascular endothelial cells (HPMECs) were cultured with DMEM media or TCM for 72 hours. The proliferation of HPMECs was continuously detected with CCK-8 kit at an interval of 12 hours. HPMECs were also injured by lipopolysaccharide, and cultured with TCM and DMEM respectively, and the tube formation capacity was detected.</p><p><strong>Results: </strong>Telocytes were positive for CD34, c-kit and vimentin. The expressions of VEGF and EGF in TCM were significantly higher, the proliferation of HPMECs cultured with TCM significantly increased, and the tube formation of HPMECs injured by endotoxin was improved with the culture of TCM, as compared with the culture of DMEM.</p><p><strong>Conclusion: </strong>The present study provides the evidence that human lung telocytes could produce the growth factors, such as VEGF and EGF. Telocytes conditional media induced the proliferation of pulmonary endothelial cells and prevented from endotoxin-induced compromise of pulmonary endothelial angiogenesis.</p>","PeriodicalId":90271,"journal":{"name":"Molecular and cellular therapies","volume":"2 ","pages":"3"},"PeriodicalIF":0.0000,"publicationDate":"2014-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452074/pdf/","citationCount":"0","resultStr":"{\"title\":\"Human lung telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro.\",\"authors\":\"Yonghua Zheng, Xiaoke Chen, Mengjia Qian, Miaomiao Zhang, Ding Zhang, Chunxue Bai, Qun Wang, Xiangdong Wang\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>In the previous studies, telocytes were found near the capillaries in many tissues, especially on the extracellular matrix of blood vessels and positive to CD34 and c-kit. Therefore, the present study aimed to explore if telocytes could produce angiogenesis associated cytokines, promote the proliferation and the angiogenesis of vascular endothelial cells in vitro.</p><p><strong>Methods: </strong>Human lung telocytes were isolated and cultured, and were identified by immunofluorescence cytochemistry with CD34, c-kit and vimentin. Telocytes conditional media (TCM) was prepared, and the expressions of angiogenesis associated cytokines in TCM were detected by ELISA. Human pulmonary microvascular endothelial cells (HPMECs) were cultured with DMEM media or TCM for 72 hours. The proliferation of HPMECs was continuously detected with CCK-8 kit at an interval of 12 hours. HPMECs were also injured by lipopolysaccharide, and cultured with TCM and DMEM respectively, and the tube formation capacity was detected.</p><p><strong>Results: </strong>Telocytes were positive for CD34, c-kit and vimentin. The expressions of VEGF and EGF in TCM were significantly higher, the proliferation of HPMECs cultured with TCM significantly increased, and the tube formation of HPMECs injured by endotoxin was improved with the culture of TCM, as compared with the culture of DMEM.</p><p><strong>Conclusion: </strong>The present study provides the evidence that human lung telocytes could produce the growth factors, such as VEGF and EGF. Telocytes conditional media induced the proliferation of pulmonary endothelial cells and prevented from endotoxin-induced compromise of pulmonary endothelial angiogenesis.</p>\",\"PeriodicalId\":90271,\"journal\":{\"name\":\"Molecular and cellular therapies\",\"volume\":\"2 \",\"pages\":\"3\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2014-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4452074/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular and cellular therapies\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2014/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular and cellular therapies","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2014/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

背景:在以往的研究中,在许多组织的毛细血管附近发现了远端细胞,特别是在血管的细胞外基质上,CD34和c-kit呈阳性。因此,本研究旨在探讨远端细胞是否能在体外产生血管生成相关细胞因子,促进血管内皮细胞的增殖和血管生成。方法:分离培养人肺远端细胞,用CD34、c-kit和vimentin进行免疫荧光细胞化学鉴定。制备远端细胞条件培养基(TCM),采用ELISA法检测TCM中血管生成相关细胞因子的表达。人肺微血管内皮细胞(hpmes)分别用DMEM培养基或中药培养72小时。CCK-8试剂盒每隔12小时连续检测hpmes的增殖情况。脂多糖对hpmec进行损伤,分别用中药和DMEM培养,检测其成管能力。结果:远端细胞CD34、c-kit、vimentin阳性。与DMEM培养相比,中药培养的内皮生长因子(VEGF)和表皮生长因子(EGF)表达显著升高,中药培养的hpmes增殖显著增加,内毒素损伤hpmes的管状形成改善。结论:本研究提供了人肺远端细胞能够产生VEGF、EGF等生长因子的证据。远端细胞条件培养基诱导肺内皮细胞增殖,阻止内毒素诱导的肺内皮血管生成损害。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Human lung telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro.

Human lung telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro.

Human lung telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro.

Human lung telocytes could promote the proliferation and angiogenesis of human pulmonary microvascular endothelial cells in vitro.

Background: In the previous studies, telocytes were found near the capillaries in many tissues, especially on the extracellular matrix of blood vessels and positive to CD34 and c-kit. Therefore, the present study aimed to explore if telocytes could produce angiogenesis associated cytokines, promote the proliferation and the angiogenesis of vascular endothelial cells in vitro.

Methods: Human lung telocytes were isolated and cultured, and were identified by immunofluorescence cytochemistry with CD34, c-kit and vimentin. Telocytes conditional media (TCM) was prepared, and the expressions of angiogenesis associated cytokines in TCM were detected by ELISA. Human pulmonary microvascular endothelial cells (HPMECs) were cultured with DMEM media or TCM for 72 hours. The proliferation of HPMECs was continuously detected with CCK-8 kit at an interval of 12 hours. HPMECs were also injured by lipopolysaccharide, and cultured with TCM and DMEM respectively, and the tube formation capacity was detected.

Results: Telocytes were positive for CD34, c-kit and vimentin. The expressions of VEGF and EGF in TCM were significantly higher, the proliferation of HPMECs cultured with TCM significantly increased, and the tube formation of HPMECs injured by endotoxin was improved with the culture of TCM, as compared with the culture of DMEM.

Conclusion: The present study provides the evidence that human lung telocytes could produce the growth factors, such as VEGF and EGF. Telocytes conditional media induced the proliferation of pulmonary endothelial cells and prevented from endotoxin-induced compromise of pulmonary endothelial angiogenesis.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信