A. Lagha , S. Zidi , M. Stayoussef , E. Gazouani , R. Kochkar , S. Kochbati , W.Y. Almawi , B. Yacoubi-Loueslati
{"title":"突尼斯类风湿关节炎患者白细胞介素-1β、白细胞介素-1 - ra、白细胞介素-10和肿瘤坏死因子-α多态性","authors":"A. Lagha , S. Zidi , M. Stayoussef , E. Gazouani , R. Kochkar , S. Kochbati , W.Y. Almawi , B. Yacoubi-Loueslati","doi":"10.1016/j.patbio.2015.04.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>The aim of this study was to investigate the role of IL-1β (−511C>T), TNFα (-308 G>A), IL-10 (-1082 G<!--> <!-->><!--> <!-->A) and IL-1RN VNTR polymorphisms in the susceptibility to rheumatoid arthritis (RA) in Tunisians.</p></div><div><h3>Patients and methods</h3><p>Using PCR-based methods, 104 RA patients and 150 healthy controls were investigated. We compared allele and genotype frequencies in RA patients versus controls and analyzed their correlations with erosive form (EF).</p></div><div><h3>Results</h3><p>IL1-RN VNTR A1A3 genotype is associated with higher risk of RA (<em>P</em> <em>=</em> <!-->0.012, OR<!--> <!-->=<!--> <!-->4.31). Among the cases, males who carry this genotype were more exposed to RA (<em>P</em> <!-->=<!--> <!-->0.044, OR<!--> <!-->=<!--> <!-->8, 47). For IL1- β gene, a significantly higher frequency of the -511C/C genotype was observed in RA patients in comparison to controls (<em>P</em> <em>=</em> <!-->0.013, OR<!--> <!-->=<!--> <!-->2.45). This higher frequency was especially observed in women (<em>P</em> <!-->=<!--> <!-->0,003, OR<!--> <!-->=<!--> <!-->3.42). In contrast, IL10−1082G/G genotype was less common in patients (<em>P</em> <em>=</em> <!-->0.046, OR<!--> <!-->=<!--> <!-->0.46). According to EF, men carrying IL1-RN VNTR A1A3 (<em>P</em> <!-->=<!--> <!-->0.005 OR<!--> <!-->=<!--> <!-->5.28) and IL1-β−511C/C (<em>P</em> <!-->=<!--> <!-->0.015 OR<!--> <!-->=<!--> <!-->2.61) genotypes develop non EF of RA. Moreover, TNFα-308 A allele (<em>P</em> <!-->=<!--> <!-->0.024, OR<!--> <!-->=<!--> <!-->1.84) and A/A genotype (<em>P</em> <!-->=<!--> <!-->0.033, OR<!--> <!-->=<!--> <!-->3.1) were positively associated to EF of RA. However, G allele (<em>P</em> <!-->=<!--> <!-->0.024, OR<!--> <!-->=<!--> <!-->0.31) and GG genotype (<em>P</em> <!-->=<!--> <!-->0.31, OR<!--> <!-->=<!--> <!-->0.031) of the TNFα-308 were protectors.</p></div><div><h3>Conclusion</h3><p>Our results indicated that IL-1RN VNTR, IL-1β (−511C>T) and IL-10 (-1082 G>A) are associated with susceptibility to RA, and that IL-1RN VNTR, IL-1β (−511C>T) and TNFα (-308 G>A) are associated with severity of RA.</p></div>","PeriodicalId":19743,"journal":{"name":"Pathologie-biologie","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.patbio.2015.04.004","citationCount":"21","resultStr":"{\"title\":\"Interleukin-1β, Interleukin1-Ra, Interleukin-10, and tumor necrosis factor-α polymorphisms in Tunisian patients with rheumatoid arthritis\",\"authors\":\"A. Lagha , S. Zidi , M. Stayoussef , E. Gazouani , R. Kochkar , S. Kochbati , W.Y. Almawi , B. Yacoubi-Loueslati\",\"doi\":\"10.1016/j.patbio.2015.04.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p>The aim of this study was to investigate the role of IL-1β (−511C>T), TNFα (-308 G>A), IL-10 (-1082 G<!--> <!-->><!--> <!-->A) and IL-1RN VNTR polymorphisms in the susceptibility to rheumatoid arthritis (RA) in Tunisians.</p></div><div><h3>Patients and methods</h3><p>Using PCR-based methods, 104 RA patients and 150 healthy controls were investigated. We compared allele and genotype frequencies in RA patients versus controls and analyzed their correlations with erosive form (EF).</p></div><div><h3>Results</h3><p>IL1-RN VNTR A1A3 genotype is associated with higher risk of RA (<em>P</em> <em>=</em> <!-->0.012, OR<!--> <!-->=<!--> <!-->4.31). Among the cases, males who carry this genotype were more exposed to RA (<em>P</em> <!-->=<!--> <!-->0.044, OR<!--> <!-->=<!--> <!-->8, 47). For IL1- β gene, a significantly higher frequency of the -511C/C genotype was observed in RA patients in comparison to controls (<em>P</em> <em>=</em> <!-->0.013, OR<!--> <!-->=<!--> <!-->2.45). This higher frequency was especially observed in women (<em>P</em> <!-->=<!--> <!-->0,003, OR<!--> <!-->=<!--> <!-->3.42). In contrast, IL10−1082G/G genotype was less common in patients (<em>P</em> <em>=</em> <!-->0.046, OR<!--> <!-->=<!--> <!-->0.46). According to EF, men carrying IL1-RN VNTR A1A3 (<em>P</em> <!-->=<!--> <!-->0.005 OR<!--> <!-->=<!--> <!-->5.28) and IL1-β−511C/C (<em>P</em> <!-->=<!--> <!-->0.015 OR<!--> <!-->=<!--> <!-->2.61) genotypes develop non EF of RA. Moreover, TNFα-308 A allele (<em>P</em> <!-->=<!--> <!-->0.024, OR<!--> <!-->=<!--> <!-->1.84) and A/A genotype (<em>P</em> <!-->=<!--> <!-->0.033, OR<!--> <!-->=<!--> <!-->3.1) were positively associated to EF of RA. However, G allele (<em>P</em> <!-->=<!--> <!-->0.024, OR<!--> <!-->=<!--> <!-->0.31) and GG genotype (<em>P</em> <!-->=<!--> <!-->0.31, OR<!--> <!-->=<!--> <!-->0.031) of the TNFα-308 were protectors.</p></div><div><h3>Conclusion</h3><p>Our results indicated that IL-1RN VNTR, IL-1β (−511C>T) and IL-10 (-1082 G>A) are associated with susceptibility to RA, and that IL-1RN VNTR, IL-1β (−511C>T) and TNFα (-308 G>A) are associated with severity of RA.</p></div>\",\"PeriodicalId\":19743,\"journal\":{\"name\":\"Pathologie-biologie\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.patbio.2015.04.004\",\"citationCount\":\"21\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pathologie-biologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0369811415000474\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pathologie-biologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0369811415000474","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 21
摘要
目的探讨IL-1β (- 511C>T)、tnf - α (-308 G>A)、IL-10 (-1082 G>A)和IL-1RN VNTR多态性在突尼斯人对类风湿关节炎(RA)的易感性。患者与方法采用pcr方法对104例RA患者和150名健康对照者进行调查。我们比较了RA患者与对照组的等位基因和基因型频率，并分析了它们与糜烂形式(EF)的相关性。结果sil1 - rn VNTR A1A3基因型与RA发病风险增高相关(P = 0.012, OR = 4.31)。在病例中，携带该基因型的男性更容易暴露于RA (P = 0.044, OR = 8,47)。对于il - 1- β基因，RA患者中- 511c /C基因型的频率明显高于对照组(P = 0.013, OR = 2.45)。这种较高的频率在女性中尤其明显(P = 0.003, OR = 3.42)。相比之下，IL10−1082G/G基因型在患者中较少见(P = 0.046, OR = 0.46)。根据EF，携带il - 1- rn VNTR A1A3 (P = 0.005 OR = 5.28)和il -β - 511C/C (P = 0.015 OR = 2.61)基因型的男性患RA非EF。TNFα-308 A等位基因(P = 0.024, OR = 1.84)和A/A基因型(P = 0.033, OR = 3.1)与RA EF呈正相关。而TNFα-308的G等位基因(P = 0.024, OR = 0.31)和GG基因型(P = 0.31, OR = 0.031)是保护因子。结论IL-1RN VNTR、IL-1β (- 511C>T)和IL-10 (-1082 G>A)与RA易感性相关，IL-1RN VNTR、IL-1β (- 511C>T)和TNFα (-308 G>A)与RA严重程度相关。
Interleukin-1β, Interleukin1-Ra, Interleukin-10, and tumor necrosis factor-α polymorphisms in Tunisian patients with rheumatoid arthritis
Objectives
The aim of this study was to investigate the role of IL-1β (−511C>T), TNFα (-308 G>A), IL-10 (-1082 G > A) and IL-1RN VNTR polymorphisms in the susceptibility to rheumatoid arthritis (RA) in Tunisians.
Patients and methods
Using PCR-based methods, 104 RA patients and 150 healthy controls were investigated. We compared allele and genotype frequencies in RA patients versus controls and analyzed their correlations with erosive form (EF).
Results
IL1-RN VNTR A1A3 genotype is associated with higher risk of RA (P= 0.012, OR = 4.31). Among the cases, males who carry this genotype were more exposed to RA (P = 0.044, OR = 8, 47). For IL1- β gene, a significantly higher frequency of the -511C/C genotype was observed in RA patients in comparison to controls (P= 0.013, OR = 2.45). This higher frequency was especially observed in women (P = 0,003, OR = 3.42). In contrast, IL10−1082G/G genotype was less common in patients (P= 0.046, OR = 0.46). According to EF, men carrying IL1-RN VNTR A1A3 (P = 0.005 OR = 5.28) and IL1-β−511C/C (P = 0.015 OR = 2.61) genotypes develop non EF of RA. Moreover, TNFα-308 A allele (P = 0.024, OR = 1.84) and A/A genotype (P = 0.033, OR = 3.1) were positively associated to EF of RA. However, G allele (P = 0.024, OR = 0.31) and GG genotype (P = 0.31, OR = 0.031) of the TNFα-308 were protectors.
Conclusion
Our results indicated that IL-1RN VNTR, IL-1β (−511C>T) and IL-10 (-1082 G>A) are associated with susceptibility to RA, and that IL-1RN VNTR, IL-1β (−511C>T) and TNFα (-308 G>A) are associated with severity of RA.
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