将活性中心掺入金刚烷基分子,构建具有溶剂依赖性催化行为的新型客仿生谷胱甘肽过氧化物酶。

Bioorganicheskaia khimiia Pub Date : 2014-03-01
Yanzhen Yin, Chao Lang, Xiaoxi Hua, Zhongfeng Shia, Yun Wang, Shufei Jiao, Chengxiang Cai, Junqiu Liu
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引用次数: 0

摘要

合成了一种新型的仿生谷胱甘肽过氧化物酶(3,3'-tellurobis(丙烷-3,1-二基)双adamantanecarboxylate,标记为ADA-Te-ADA)。ADA-Te-ADA的功能克服了巨分子仿生酶构建单元的缺点。为了揭示疏水ADA-Te-ADA的催化性能,采用pH 7.0的PBS(磷酸盐缓冲液)研究了ADA-Te-ADA的催化机理。50 mM) /甲醇溶剂混合物作为测定溶液。饱和动力学测试表明ADA-Te-ADA具有典型的酶催化行为。重要的是,ADA-Te-ADA表现出典型的溶剂依赖性催化行为。当PBs:甲醇体积比为5:5时,催化率最高,为4.29µM x min-1。特别是,基于各种底物的催化速率证明了ADA-Te-ADA略微表现出特殊的底物选择性,这是巨超分子仿生酶理想的催化表征材料。ADA-Te-ADA的成功合成对了解疏水客体仿生谷胱甘肽过氧化物酶的催化机理具有重要意义。同时也为构建大型超分子仿生酶提供了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Construction of a novel guest biomimetic glutathione peroxidase with solvent-dependent catalytic behavior by incorporating the active center into adamantyl molecule.

A novel guest biomimetic glutathione peroxidase (3,3'-tellurobis(propane-3,1-diyl) diadamantanecarboxylate, denoted as ADA-Te-ADA) was synthesized. ADA-Te-ADA functioned to overcome the disadvantages in the construction of building block for giant supramolecular biomimetic enzyme. To reveal the catalytic property of hydrophobic ADA-Te-ADA, the catalytic mechanism was investigated using PBS (phosphate buffer (pH 7.0. 50 mM))/methanol solvent mixture as assay solution. Itindicated that ADA-Te-ADA exhibited typical enzyme catalytic behavior by saturation kinetics measurement. Importantly, ADA-Te-ADA exhibited the typical solvent-dependent catalytic behavior. And the highest catalytic rate 4.29 µM x min-1 was obtained when the volume ratio of PBs: methanol was 5 : 5. Especially, the catalytic rates obtained based on various substrates proved that ADA-Te-ADA slightly displayed special substrate selectivity, which was the ideal catalytic characterization of building block for giant supramolecular biomimetic enzyme. The successfully synthesis of ADA-Te-ADA might highlight for the understanding of the catalytic mechanism of hydrophobic guest biomimetic glutathione peroxidase. And it also might provide the basement for the construction of giant supramolecular biomimetic enzyme.

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