宿主红细胞环境影响输出蛋白2的定位,这是疟原虫转位的重要组成部分。

Eukaryotic Cell Pub Date : 2015-04-01 Epub Date: 2015-02-06 DOI:10.1128/EC.00228-14
Elamaran Meibalan, Mary Ann Comunale, Ana M Lopez, Lawrence W Bergman, Anand Mehta, Akhil B Vaidya, James M Burns
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引用次数: 18

摘要

在红细胞内复制的疟疾寄生虫将大量蛋白质输出到红细胞细胞质中,以促进寄生虫的生长和生存。PTEX是疟原虫编码的转位子,在恶性疟原虫感染的红细胞中介导蛋白在寄生液泡膜(PVM)上的转运。输出到红血球细胞质的蛋白质已定位于膜结构,如毛雷尔裂隙、小泡和管泡网络。间日疟原虫感染网织红细胞中蛋白质运输的可比研究有限。在约利疟原虫感染的网织红细胞中,我们在推测通过PVM转运的蛋白质组学筛选中鉴定了输出蛋白2 (Exp2)。免疫荧光研究表明,P. yoelii Exp2 (PyExp2)主要定位于PVM。出乎意料的是,PyExp2也与网状细胞细胞质中独特的膜结合囊泡有关。这与成熟红细胞中的恶性疟原虫形成对比,在成熟红细胞中,恶性疟原虫Exp2 (PfExp2)仅局限于PVM。P. yoelii输出的两个蛋白PY04481(由pyst-a基因编码)和PY06203 (PypAg-1)与这些pyexp2阳性囊泡部分共定位。进一步分析显示,在约氏疟原虫、伯氏疟原虫和恶性疟原虫中,细胞质exp2阳性囊泡主要在CD71(+)网织红细胞和成熟红细胞中观察到。在转基因yoelii 17X寄生虫中,血凝素标记的PyExp2基因与PVM和细胞质囊泡的关联被保留,但PyExp2基因难以缺失。这些数据表明,Exp2在小鼠和人红细胞中的定位可能受到宿主细胞环境的影响。Exp2可能在寄生虫将蛋白质运送到网织细胞细胞质的多个通路上发挥作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Host erythrocyte environment influences the localization of exported protein 2, an essential component of the Plasmodium translocon.

Host erythrocyte environment influences the localization of exported protein 2, an essential component of the Plasmodium translocon.

Host erythrocyte environment influences the localization of exported protein 2, an essential component of the Plasmodium translocon.

Host erythrocyte environment influences the localization of exported protein 2, an essential component of the Plasmodium translocon.

Malaria parasites replicating inside red blood cells (RBCs) export a large subset of proteins into the erythrocyte cytoplasm to facilitate parasite growth and survival. PTEX, the parasite-encoded translocon, mediates protein transport across the parasitophorous vacuolar membrane (PVM) in Plasmodium falciparum-infected erythrocytes. Proteins exported into the erythrocyte cytoplasm have been localized to membranous structures, such as Maurer's clefts, small vesicles, and a tubovesicular network. Comparable studies of protein trafficking in Plasmodium vivax-infected reticulocytes are limited. With Plasmodium yoelii-infected reticulocytes, we identified exported protein 2 (Exp2) in a proteomic screen of proteins putatively transported across the PVM. Immunofluorescence studies showed that P. yoelii Exp2 (PyExp2) was primarily localized to the PVM. Unexpectedly, PyExp2 was also associated with distinct, membrane-bound vesicles in the reticulocyte cytoplasm. This is in contrast to P. falciparum in mature RBCs, where P. falciparum Exp2 (PfExp2) is exclusively localized to the PVM. Two P. yoelii-exported proteins, PY04481 (encoded by a pyst-a gene) and PY06203 (PypAg-1), partially colocalized with these PyExp2-positive vesicles. Further analysis revealed that with P. yoelii, Plasmodium berghei, and P. falciparum, cytoplasmic Exp2-positive vesicles were primarily observed in CD71(+) reticulocytes versus mature RBCs. In transgenic P. yoelii 17X parasites, the association of hemagglutinin-tagged PyExp2 with the PVM and cytoplasmic vesicles was retained, but the pyexp2 gene was refractory to deletion. These data suggest that the localization of Exp2 in mouse and human RBCs can be influenced by the host cell environment. Exp2 may function at multiple points in the pathway by which parasites traffic proteins into and through the reticulocyte cytoplasm.

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来源期刊
Eukaryotic Cell
Eukaryotic Cell 生物-微生物学
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审稿时长
1 months
期刊介绍: Eukaryotic Cell (EC) focuses on eukaryotic microbiology and presents reports of basic research on simple eukaryotic microorganisms, such as yeasts, fungi, algae, protozoa, and social amoebae. The journal also covers viruses of these organisms and their organelles and their interactions with other living systems, where the focus is on the eukaryotic cell. Topics include: - Basic biology - Molecular and cellular biology - Mechanisms, and control, of developmental pathways - Structure and form inherent in basic biological processes - Cellular architecture - Metabolic physiology - Comparative genomics, biochemistry, and evolution - Population dynamics - Ecology
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