抗菌肽hepcidin对结核患者铁超载的影响。

Mina Javaheri-Kermani, Touraj Farazmandfar, Abolghasem Ajami, Yaghoub Yazdani
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引用次数: 16

摘要

背景:铁的获取对结核分枝杆菌的生长至关重要。Hepcidin是一种抗菌肽,也是先天免疫反应的一个组成部分。Hepcidin体外抑制结核分枝杆菌生长。在这项研究中,我们决定在结核病患者中鉴定HAMP启动子的-582A> G变异,并研究其对血清铁、铁蛋白和hepcidin水平的影响。方法:对105例结核病患者和104例健康人进行抽样调查。采用四引物PCR对-582A> G多态性进行基因分型。采用ELISA试剂盒检测血清hepcidin水平。采用SPSS软件进行统计分析。结果:G等位基因与结核病有显著相关性(95%置信区间= 2-4.8,p < 0.000)。在-582A>G多态性基因型之间,血清铁和hepcidin水平有显著差异,但铁蛋白水平无显著差异。hepcidin与铁呈显著负相关(r = -0.849, p = 0.006)。结论:TB患者血清hepcidin水平与HAMP -582A> G变异高度相关。这些观察结果表明这种多态性在铁代谢中的假设作用。Hepcidin可能是治疗结核病的一种选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of hepcidin antimicrobial peptide on iron overload in tuberculosis patients.

Background: Iron acquisition is essential for the growth of Mycobacterium tuberculosis. Hepcidin is known as an antimicrobial peptide and a component of the innate immune response. Hepcidin inhibits M. tuberculosis growth in vitro. In this study, we decided to identify -582A> G variants of the HAMP promoter in patients with tuberculosis (TB) and investigate its effect on serum iron, ferritin, and hepcidin levels.

Methods: The sample population consisted of 105 patients with TB and 104 healthy individuals. The -582A> G polymorphism was genotyped using a tetra-primers PCR set. Serum levels of hepcidin were determined using an ELISA kit. Statistical analysis was performed using SPSS software.

Results: The G allele is meaningfully associated with TB disease (95% confidence interval = 2-4.8, p < 0.000). Significant differences were seen in the levels of serum iron and hepcidin but not ferritin between the -582A>G polymorphism genotypes. There was significant reverse correlation between hepcidin and iron (r = -0.849, p = 0.006).

Conclusion: A high association was found between serum hepcidin levels and the HAMP -582A> G variants in patients with TB. These observations indicate a hypothetical role of this polymorphism in iron metabolism. Hepcidin could perhaps be an option for the treatment of TB.

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