噬菌体HK022 Nun蛋白在转录延伸过程中通过阻断RNA聚合酶的横向迁移来阻止转录。

Bacteriophage Pub Date : 2014-07-30 eCollection Date: 2014-01-01 DOI:10.4161/bact.32187
Christal L Vitiello, Max E Gottesman
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引用次数: 3

摘要

噬菌体HK022通过破坏λ抗终止系统和阻止λ染色体上的转录而排斥噬菌体λ。12 kDa的HK022 Nun蛋白通过其N端Arginine Rich Motif (ARM)结合到λ新生转录物上,阻断λ N的进入,并通过与RNA聚合酶的c端相互作用阻止转录。在纯化的体外系统中,我们最近证明了Nun通过限制转录延伸复合体(TEC)沿DNA寄存器的横向运动来阻止转录,从而冻结转运状态。我们将讨论导致这一结论的一些关键实验,并提出进一步支持这一结论的其他结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Bacteriophage HK022 Nun protein arrests transcription by blocking lateral mobility of RNA polymerase during transcription elongation.

Bacteriophage HK022 Nun protein arrests transcription by blocking lateral mobility of RNA polymerase during transcription elongation.

Bacteriophage HK022 Nun protein arrests transcription by blocking lateral mobility of RNA polymerase during transcription elongation.

Bacteriophage HK022 Nun protein arrests transcription by blocking lateral mobility of RNA polymerase during transcription elongation.

Coliphage HK022 excludes phage λ by subverting the λ antitermination system and arresting transcription on the λ chromosome. The 12 kDa HK022 Nun protein binds to λ nascent transcript through its N-terminal Arginine Rich Motif (ARM), blocking access by λ N and arresting transcription via a C-terminal interaction with RNA polymerase. In a purified in vitro system, we recently demonstrated that Nun arrests transcription by restricting lateral movement of transcription elongation complex (TEC) along the DNA register, thereby freezing the translocation state. We will discuss some of the key experiments that led to this conclusion, as well as present additional results that further support it.

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