球上两个活性位点的扩散控制反应速率。

Q1 Biochemistry, Genetics and Molecular Biology
BMC Biophysics Pub Date : 2014-06-04 eCollection Date: 2014-01-01 DOI:10.1186/2046-1682-7-3
David E Shoup
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引用次数: 10

摘要

背景:将均匀球形反应物的扩散限制反应速率推广到各向异性反应。以前的工作表明,均匀球的蛋白质模型在许多情况下是不令人满意的。结合两个活性位点的配体在一个球形酶或细胞上的竞争进行了分析研究。结果:给出了目标物质两端两个位点的反应速率常数。这与单个位点的反应速率的两倍相比较。研究发现,位点之间的竞争比两个位点单独的反应速率要低。网站之间的竞争不显现,直到网站的半角大于30度。结论:在网站规模变大之前,网站之间的竞争可以忽略不计。竞争效应随着θ变大而增强。当θ = pi/2时,给出了最大的效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Diffusion-controlled reaction rates for two active sites on a sphere.

Diffusion-controlled reaction rates for two active sites on a sphere.

Diffusion-controlled reaction rates for two active sites on a sphere.

Background: The diffusion-limited reaction rate of a uniform spherical reactant is generalized to anisotropic reactivity. Previous work has shown that the protein model of a uniform sphere is unsatisfactory in many cases. Competition of ligands binding to two active sites, on a spherical enzyme or cell is studied analytically.

Results: The reaction rate constant is given for two sites at opposite ends of the species of interest. This is compared with twice the reaction rate for a single site. It is found that the competition between sites lowers the reaction rate over what is expected for two sites individually. Competition between sites does not show up, until the site half angle is greater than 30 degrees.

Conclusions: Competition between sites is negligible until the site size becomes large. The competitive effect grows as theta becomes large. The maximum effect is given for theta = pi/2.

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BMC Biophysics
BMC Biophysics BIOPHYSICS-
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